PC-1 enhances c-myc gene expression in prostate cancer cells

Lan Yu, Qing Guo Shi, Xiao Long Qian, Shan Hu Li, Hong Tao Wang, Le Le Wang, Jian Guang Zhou

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

PC-1(Prostate and colon gene 1) gene belongs to TPD52 (Tumor Protein D52) gene family. The expression of PC-1 is found to promote androgen-independent progression. This study was conducted to assess the mechnism of promotion of androgen-independent progression in PC-1 gene. The c-myc gene expression was tested by RT-PCR and Western blotting analyses in the LNCaP-pc-1 and LNCaP-zero cell line. After separation of cytoplasm and nulear proteins of the LNCaP-pc-1 and LNCaP-zero cell line, the beta-catenin protein was detected by Western blotting. C4-2 cell line was used to examine the effects of 10058-F4 on the PC-1 gene expression. The results of RT-PCR and Western blotting indicated that PC-1 enhanced c-myc gene expression in prostate cancer cells, PC-1 was also found to enhance beta-catenin expression in nuclear. Furthermore, a small-molecule c-Myc inhibitor, 10058-F4 represses PC-1 gene expression in C4-2 cell line. Our findings suggest that PC-1 enhances c-myc gene expression in prostate cancer cells through the Wnt/beta-catenin pathway. Meanwhile, c-myc plays a feed-forward role in enhancing PC-1 driven c-myc gene expression, and promotes prostate an-drogen-independent progression.

Original languageEnglish (US)
Pages (from-to)348-352
Number of pages5
JournalYi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji
Volume32
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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