Pdgfrβ+ mural preadipocytes contribute to adipocyte hyperplasia induced by high-fat-diet feeding and prolonged cold exposure in adult mice

Lavanya Vishvanath, Karen A. Macpherson, Chelsea Hepler, Qiong A. Wang, Mengle Shao, Stephen B. Spurgin, Margaret Y. Wang, Christine M. Kusminski, Thomas S. Morley, Rana K. Gupta

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The expansion of white adipose tissue (WAT) in obesity involves de novo differentiation of new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize Zfp423GFP reporter mice to characterize adipose mural (Pdgfrβ+) cells with varying levels of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like mural cells. Using our "MuralChaser" lineage tracking system, we uncover adipose perivascular cells as developmental precursors of adipocytes formed in obesity, with adipogenesis and precursor abundance regulated in a depot-dependent manner. Interestingly, Pdgfrβ+ cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. These results provide genetic evidence for a mural cell origin of white adipocytes in obesity and suggest that beige adipogenesis may originate from multiple sources.

Original languageEnglish (US)
Pages (from-to)350-359
Number of pages10
JournalCell Metabolism
Volume23
Issue number2
DOIs
StatePublished - Feb 9 2016

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High Fat Diet
Adipocytes
Hyperplasia
Adipogenesis
White Adipose Tissue
Obesity
White Adipocytes
Adipose Tissue
Population

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Pdgfrβ+ mural preadipocytes contribute to adipocyte hyperplasia induced by high-fat-diet feeding and prolonged cold exposure in adult mice. / Vishvanath, Lavanya; Macpherson, Karen A.; Hepler, Chelsea; Wang, Qiong A.; Shao, Mengle; Spurgin, Stephen B.; Wang, Margaret Y.; Kusminski, Christine M.; Morley, Thomas S.; Gupta, Rana K.

In: Cell Metabolism, Vol. 23, No. 2, 09.02.2016, p. 350-359.

Research output: Contribution to journalArticle

Vishvanath, Lavanya ; Macpherson, Karen A. ; Hepler, Chelsea ; Wang, Qiong A. ; Shao, Mengle ; Spurgin, Stephen B. ; Wang, Margaret Y. ; Kusminski, Christine M. ; Morley, Thomas S. ; Gupta, Rana K. / Pdgfrβ+ mural preadipocytes contribute to adipocyte hyperplasia induced by high-fat-diet feeding and prolonged cold exposure in adult mice. In: Cell Metabolism. 2016 ; Vol. 23, No. 2. pp. 350-359.
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