@article{aa8a382cd089421fa6c8abdf1da9c7ad,
title = "PDZ proteins bind, cluster, and synaptically colocalize with Eph receptors and their ephrin ligands",
abstract = "Localizing cell surface receptors to specific subcellular positions can be critical for their proper functioning, as most notably demonstrated at neuronal synapses. PDZ proteins apparently play critical roles in such protein localizations. Receptor tyrosine kinases have not been previously shown to interact with PDZ proteins in vertebrates. We report that Eph receptors and their membrane-linked ligands all contain PDZ recognition motifs and can bind and be clustered by PDZ proteins. In addition, we find that Eph receptors and ligands colocalize with PDZ proteins at synapses. Thus, PDZ proteins may play critical roles in localizing vertebrate receptor tyrosine kinases and/or their ligands and may be particularly important for Eph function in guidance or patterning or at the synapse.",
author = "Richard Torres and Firestein, {Bonnie L.} and Hualing Dong and Jeff Staudinger and Olson, {Eric N.} and Huganir, {Richard L.} and Bredt, {David S.} and Gale, {Nicholas W.} and Yancopoulos, {George D.}",
note = "Funding Information: We thank Drs. L. P. Schleifer and P. Roy Vagelos for a stimulating scientific environment and many Regeneron scientists for discussions, advice, and reagents. We also thank Dr. R. A. DePinho for stimulating discussions at the inception of this project and for his support, D. S. Hintz and L. T. Esau for expert technical assistance, Drs. P. Hickmott and P. Steen for excellent technical help with the confocal imaging, and C. R. Murphy and E. J. Burrows for excellent graphics work. We thank S. J. Holland and T. Pawson for the EphB2 antibody, R. A. DePinho for the cask plasmid, J. Trimmer for advice with the COS7 clustering assay, and S. M. Hollenberg for yeast two-hybrid plasmids and the L40 yeast strain. B. L. F and D. S. B. thank J. F. Kilbridge for expert technical assistance. B. L. F. is supported by a National Institutes of Health National Research Service Award. D. S. B. is supported by the National Institutes of Health, the Searles Scholars Program, the Culpeper Foundation, the Beckman Foundation, and the Lucille P. Markey Charitable Trust. E. N. O is supported by the National Institutes of Health and the Robert A. Welch Foundation. ",
year = "1998",
month = dec,
doi = "10.1016/S0896-6273(00)80663-7",
language = "English (US)",
volume = "21",
pages = "1453--1463",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}