Pediatric oligodendrogliomas: A study of molecular alterations on 1p and 19q using fluorescence in situ hybridization

Ravi Raghavan, Jyoti Balani, Arie Perry, Linda Margraf, Mary B. Vono, Dan X. Cai, Robert E. Wyatt, Elisabeth J. Rushing, Daniel C. Bowers, Linda S. Hynan, Charles L. White

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Oligodendrogliomas (OGs) are rare in children and have not been well characterized from a molecular viewpoint. In adults, losses on chromosomes 1p and/or 19q are common in "oligodendroglial" neoplasms and are highly associated with chemosensitivity and greater length of survival, especially in the anaplastic category. We have analyzed the 1p/19q status of pediatric OGs and compared it with similar alterations in adult OGs. Paraffin sections from 26 pediatric OGs (21 WHO Grade II OGs; 2 anaplastic oligodendrogliomas [AOGs]; and 3 mixed oligo-astrocytomas [MOA]) were retrieved. Fluorescence in situ hybridization (FISH) was performed using probes spanning the 1p32 and 19q13 regions. In tumors from children 0 to 9 years of age (n = 15), none had any deletions on 1p or 19q, but 2 had polysomies for 1p and/or 19q. All are alive and 4 have had recurrences. In tumors from children >9 years, losses were identified on chromosomes 1p (5/11: 45%) and/or 19q (3/11; 27%), but to a much lesser extent than that observed in adult OGs. Tumors from 6 older patients also had polysomies for 1p and/or 19q. Although the majority of the older children are alive, 4 had recurrences. Curiously, 2 of the older children with AOGs had combined losses and polysomies on 1p and 19q, but responded poorly to treatment and died within a year. We conclude that alterations on 1p or 19q are infrequent in pediatric compared to adult OGs and are virtually absent in OGs presenting in the first decade of life. Compared to adults therefore, different genetic pathways are likely involved in the pathogenesis of most pediatric OGs. Genomic screening on a larger series is clearly indicated to delineate the unique molecular characteristics of these rare pediatric tumors.

Original languageEnglish (US)
Pages (from-to)530-537
Number of pages8
JournalJournal of neuropathology and experimental neurology
Volume62
Issue number5
DOIs
StatePublished - May 1 2003

Keywords

  • Chromosome 19q
  • Chromosome 1p
  • Fluorescence in situ hybridization (FISH)
  • Molecular genetics
  • Pediatric oligodendroglioma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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