Pentobarbital modifies the lipid raft-protein interaction: A first clue about the anesthesia mechanism on NMDA and GABAA receptors

Francisco Javier Sierra-Valdez, J. C. Ruiz-Suárez, Ilse Delint-Ramirez

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABA A receptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.

Original languageEnglish (US)
Pages (from-to)2603-2610
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1858
Issue number11
DOIs
StatePublished - Nov 1 2016

Keywords

  • Anesthesia
  • Calorimetry
  • Lipid membrane
  • Receptors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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