Pentobarbital modifies the lipid raft-protein interaction

A first clue about the anesthesia mechanism on NMDA and GABAA receptors

Francisco Javier Sierra-Valdez, J. C. Ruiz-Suárez, Ilse Delint-Ramirez

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have shown that anesthetic agents alter the physical properties of lipid rafts on model membranes. However, if this destabilization occurs in brain membranes, altering the lipid raft-protein interaction, remains unknown. We analyzed the effects produced by pentobarbital (PB) on brain plasma membranes and lipid rafts in vivo. We characterized for the first time the thermotropic behavior of plasma membranes, synaptosomes, and lipid rafts from rat brain. We found that the transition temperature from the ordered gel to disordered liquid phase of lipids is close to physiological temperature. We then studied the effect of PB on protein composition of lipid rafts. Our results show a reduction of the total protein associated to rafts, with a higher reduction of the NMDAR compared to the GABA A receptor. Both receptors are considered the main targets of PB. In general, our results suggest that lipid rafts could be plausible mediators in anesthetic action.

Original languageEnglish (US)
Pages (from-to)2603-2610
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1858
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

Pentobarbital
GABA-A Receptors
N-Methyl-D-Aspartate Receptors
Membrane Lipids
Anesthesia
Lipids
Anesthetics
Brain
Proteins
Cell Membrane
Cell membranes
Synaptosomes
Transition Temperature
Membranes
Gels
Temperature
Rats
Physical properties
Liquids
Chemical analysis

Keywords

  • Anesthesia
  • Calorimetry
  • Lipid membrane
  • Receptors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Pentobarbital modifies the lipid raft-protein interaction : A first clue about the anesthesia mechanism on NMDA and GABAA receptors. / Sierra-Valdez, Francisco Javier; Ruiz-Suárez, J. C.; Delint-Ramirez, Ilse.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1858, No. 11, 01.11.2016, p. 2603-2610.

Research output: Contribution to journalArticle

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