PEPCK-C reexpression in the liver counters neonatal hypoglycemia in Pck1del/del mice, unmasking role in non-gluconeogenic tissues

Jana Semakova, Petra Hyroššová, Andrés Méndez-Lucas, Ernest Cutz, Jordi Bermudez, Shawn Burgess, Soledad Alcántara, José C. Perales

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Whole body cytosolic phosphoenolpyruvate carboxykinase knockout (PEPCK-C KO) mice die early after birth with profound hypoglycemia therefore masking the role of PEPCK-C in adult, non-gluconeogenic tissues where it is expressed. To investigate whether PEPCK-C deletion in the liver was critically responsible for the hypoglycemic phenotype, we reexpress this enzyme in the liver of PEPCK-C KO pups by early postnatal administration of PEPCK-C-expressing adenovirus. This maneuver was sufficient to partially rescue hypoglycemia and allow the pups to survive and identifies the liver as a critical organ, and hypoglycemia as the critical pathomechanism, leading to early postnatal death in the whole-body PEPCK-C knockout mice. Pathology assessment of survivors also suggest a possible role for PEPCK-C in lung maturation and muscle metabolism.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Physiology and Biochemistry
DOIs
StateAccepted/In press - Oct 26 2016

Fingerprint

Hypoglycemia
Liver
Phosphoenolpyruvate
Tissue
Knockout Mice
Pathology
Hypoglycemic Agents
Metabolism
Adenoviridae
Muscle
Parturition
Phenotype
Muscles
Lung
Enzymes

Keywords

  • Gluconeogenesis
  • Hepatic lipidosis
  • KO
  • Liver
  • Neonatal hypoglycemia
  • PEPCK

ASJC Scopus subject areas

  • Physiology
  • Biochemistry

Cite this

PEPCK-C reexpression in the liver counters neonatal hypoglycemia in Pck1del/del mice, unmasking role in non-gluconeogenic tissues. / Semakova, Jana; Hyroššová, Petra; Méndez-Lucas, Andrés; Cutz, Ernest; Bermudez, Jordi; Burgess, Shawn; Alcántara, Soledad; Perales, José C.

In: Journal of Physiology and Biochemistry, 26.10.2016, p. 1-10.

Research output: Contribution to journalArticle

Semakova, Jana ; Hyroššová, Petra ; Méndez-Lucas, Andrés ; Cutz, Ernest ; Bermudez, Jordi ; Burgess, Shawn ; Alcántara, Soledad ; Perales, José C. / PEPCK-C reexpression in the liver counters neonatal hypoglycemia in Pck1del/del mice, unmasking role in non-gluconeogenic tissues. In: Journal of Physiology and Biochemistry. 2016 ; pp. 1-10.
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AU - Cutz, Ernest

AU - Bermudez, Jordi

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