Perfluorocarbon imaging in vivo: A 19F MRI study in tumor-bearing mice

Ralph P. Mason, Peter P. Antich, Evelyn E. Babcock, Jenifer L. Gerberich, Ray L. Nunnally

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Multiresonance perfluorocarbon emulsions (Oxypherol and Fluosol-DA) were imaged in tumor-bearing mice using 19F spin-echo magnetic resonance imaging in vivo. Multiple thin-slice fluorine images free of chemical shift artifacts were obtained in 13 minutes and these were correlated with proton images obtained during the same experiment to delineate the anatomic distribution of perfluorocarbons. Sequential images were used to determine the time course of the distribution and the retention of the compounds in tumors and organs. 19F MR spectroscopy was used ex vivo to determine with high sensitivity the relative concentration of perfluorocarbons in different tissues and organs and to confirm the results obtained from imaging experiments. The fluorine images visually demonstrated the preferential localization of the perfluorocarbons in the liver and spleen; shortly after injection, the images also revealed the highly vascularized tumor-chest wall interface. Imaging and spectroscopy together showed that the perfluorocarbons were removed from the blood pool within hours and remained sequestered in tissues at later times; the highest concentrations were found in the spleen and liver, where the agents were retained without spectral changes for the duration of these studies. The perfluorocarbons accumulated within tumors at dose-dependent concentration, one to two orders of magnitude smaller than those observed in the spleen and liver.

Original languageEnglish (US)
Pages (from-to)475-485
Number of pages11
JournalMagnetic Resonance Imaging
Volume7
Issue number5
DOIs
StatePublished - Jan 1 1989

Keywords

  • F MRI
  • Fluorocarbon
  • H MRI
  • Spectroscopy, Murine tumors

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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