TY - JOUR
T1 - Period determination in the food-entrainable and methamphetamine-sensitive circadian oscillator(s)
AU - Pendergast, Julie S.
AU - Oda, Gisele A.
AU - Niswender, Kevin D.
AU - Yamazaki, Shin
PY - 2012/8/28
Y1 - 2012/8/28
N2 - Daily rhythmic processes are coordinated by circadian clocks, which are present in numerous central and peripheral tissues. In mammals, two circadian clocks, the food-entrainable oscillator (FEO) and methamphetamine-sensitive circadian oscillator (MASCO), are "black box" mysteries because their anatomical loci are unknown and their outputs are not expressed under normal physiological conditions. In the current study, the investigation of the time keeping mechanisms of the FEO and MASCO in mice with disruption of all three paralogs of the canonical clock gene, Period, revealed unique and convergent findings. We found that both the MASCO and FEO in Per1-/-/Per2-/-/Per3-/- mice are circadian oscillators with unusually short (∼21 h) periods. These data demonstrate that the canonical Period genes are involved in period determination in the FEO and MASCO, and computational modeling supports the hypothesis that the FEO and MASCO use the same timekeeping mechanism or are the same circadian oscillator. Finally, these studies identify Per1-/-/Per2-/-/Per3-/- mice as a unique tool critical to the search for the elusive anatomical location(s) of the FEO and MASCO.
AB - Daily rhythmic processes are coordinated by circadian clocks, which are present in numerous central and peripheral tissues. In mammals, two circadian clocks, the food-entrainable oscillator (FEO) and methamphetamine-sensitive circadian oscillator (MASCO), are "black box" mysteries because their anatomical loci are unknown and their outputs are not expressed under normal physiological conditions. In the current study, the investigation of the time keeping mechanisms of the FEO and MASCO in mice with disruption of all three paralogs of the canonical clock gene, Period, revealed unique and convergent findings. We found that both the MASCO and FEO in Per1-/-/Per2-/-/Per3-/- mice are circadian oscillators with unusually short (∼21 h) periods. These data demonstrate that the canonical Period genes are involved in period determination in the FEO and MASCO, and computational modeling supports the hypothesis that the FEO and MASCO use the same timekeeping mechanism or are the same circadian oscillator. Finally, these studies identify Per1-/-/Per2-/-/Per3-/- mice as a unique tool critical to the search for the elusive anatomical location(s) of the FEO and MASCO.
KW - C57BL/6J
KW - Food anticipatory activity
KW - Knockout
KW - Restricted feeding
KW - Suprachiasmatic nuclei
UR - http://www.scopus.com/inward/record.url?scp=84865564658&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865564658&partnerID=8YFLogxK
U2 - 10.1073/pnas.1206213109
DO - 10.1073/pnas.1206213109
M3 - Article
C2 - 22891330
AN - SCOPUS:84865564658
SN - 0027-8424
VL - 109
SP - 14218
EP - 14223
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 35
ER -