Between June 1991 and January 1995 we performed 67 peripheral blood progenitor cell transplants (PBPCT). Ten patients (group 1) were mobilised with 7 gm/m2 of cyclophosphamide followed by daily G-CSF injections (5 μg/kg, subcutaneously). When the white cell count reached 1 x 109/l they were leukapheresed for 5 days. After stem cell infusion they received G-CSF (10 μg/kg/day) until the neutrophil count reached 1.5 x 109/l. Fifty-six patients had PBPCs mobilised with 3 gm/m2 of cyclophosphamide followed by daily subcutaneous G-CSF (5 μg/kg) and PBPCs were harvested on 2 consecutive days, when the white cell count rose to 4 x 109/l. After stem cell infusion this group did not receive G-CSF, In 47 of the 56 patients (group 2) adequate MNC (≥ 4 x 108/kg) and/or CFU-GM (≥ 10 x 104/kg) were obtained. Insufficient MNC and/or CFU-GM were obtained in 10 patients. They were therefore transplanted using a combination of bone marrow and peripheral blood progenitor cells (group 3). Overall 64 patients successfully engrafted. Median days to neutrophils ≥ 0.5 x 109/l were 9 (range 8-13), 12 (range 8-25) and 11 (range 9-16) and to platelets ≥ 50 x 109/l were 11 (range 9-23), 13 (range 9-90) and 16 (range 13-99) in groups 1, 2 and 3 respectively. Patients in group 1 had a faster neutrophil recovery than patients in group 2 (P = 0.0002). The three patients who failed to engraft all received a combination of autologous peripheral blood and bone marrow cells.
|Original language||English (US)|
|Number of pages||5|
|Journal||Bone Marrow Transplantation|
|State||Published - Apr 1996|
- PBPC transplantation
- Single centre
ASJC Scopus subject areas