Peroxisomal proliferator-activated receptor-α-dependent inhibition of endothelial cell proliferation and tumorigenesis

Ambra Pozzi, Maria Raquel Ibanez, Arnaldo E. Gatica, Shilin Yang, Shouzuo Wei, Shaojun Mei, J R Falck, Jorge H. Capdevila

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

The peroxisomal proliferator-activated nuclear receptor-α (PPARα), the target for most hypolipidemic drugs in current clinical use, regulates the transcription of genes involved in lipid metabolism and transport, and energy homeostasis. More recently, PPARα and its ligands have been implicated in inflammatory responses and the regulation of cell proliferation. PPARα also regulates the expression of Cyp4a fatty acid ω-hydroxylases and Cyp2c arachidonic acid epoxygenase genes. To study the role of the PPARα receptor and of its Cyp2c epoxygenase gene target in tumorigenesis, we treated mice injected with tumor cells with Wy-14,643, a PPARα-selective ligand. Compared with untreated controls, Wy-14643-treated animals showed marked reductions in tumor growth and vascularization, which were accompanied by decreases in the plasma levels of pro-angiogenic epoxygenase metabolites (EETs), hepatic EET biosynthesis, and Cyp2c epoxygenase expression. All these Wy-14643-induced responses were absent in PPARα-/- mice and are thus PPARα-mediated. Primary cultures of mouse lung endothelial cells treated with Wy-14643 showed reductions in cell proliferation and in the formation of capillary-like structures. These effects were absent in cells obtained from PPRAα-/- mice and reversed by the addition of EETs. These results identify important anti-angiogenic and anti-tumorigenic roles for PPARα, characterize the contribution of its Cyp2c epoxygenases gene target to these responses, and suggest potential anti-cancer roles for this nuclear receptor and its ligands.

Original languageEnglish (US)
Pages (from-to)17685-17695
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number24
DOIs
StatePublished - Jun 15 2007

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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