Persistent expression of MNF identifies myogenic stem cells in postnatal muscles

Daniel J. Garry, Quan Yang, Rhonda Bassel-Duby, R. Sanders Williams

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Skeletal muscles contain an undifferentiated myogenic stem cell pool (satellite cells) that can be mobilized to regenerate myofibers in response to injury. We have determined that the winged helix transcription factor MNF is expressed selectively in quiescent satellite cells, which do not express known regulators of the myogenic program. Following muscle injury, MNF is present transiently in proliferating satellite cells and in centralized nuclei of regenerating myofibers, but expression declines as these fibers mature, until only the residual stem cell pool continues to express detectable levels of MNF. MNF also is expressed selectively but transiently at embryonic stages of myogenesis in the developing myotome, limb bud precursors, and heart tube, but by late fetal stages of development, MNF is down-regulated within differentiated cardiac and skeletal myocytes, and persistently high expression is observed only in satellite cells. These data identify MNF as a marker of quiescent satellite cells and suggest that downstream genes controlled by MNF serve to modulate proliferative growth or differentiation in this unique cell population.

Original languageEnglish (US)
Pages (from-to)280-294
Number of pages15
JournalDevelopmental Biology
Volume188
Issue number2
DOIs
StatePublished - Aug 15 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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