Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart

Cindy M. Martin, Annette P. Meeson, Scott M. Robertson, Thomas J. Hawke, James A. Richardson, Susan Bates, Sean C. Goetsch, Teresa D. Gallardo, Daniel J. Garry

Research output: Contribution to journalArticle

524 Citations (Scopus)

Abstract

Stem cells are important in the maintenance and repair of adult tissues. A population of cells, termed side population (SP) cells, has stem cell characteristics as they have been shown to contribute to diverse lineages. In this study, we confirm that Abcg2 is a determinant of the SP cell phenotype. Therefore, we examined Abcg2 expression during murine embryogenesis and observed robust expression in the blood islands of the E8.5 yolk sac and in developing tissues including the heart. During the latter stages of embryogenesis, Abcg2 identifies a rare cell population in the developing organs. We further establish that the adult heart contains an Abcg2 expressing SP cell population and these progenitor cells are capable of proliferation and differentiation. We define the molecular signature of cardiac SP cells and compare it to embryonic stem cells and adult cardiomyocytes using emerging technologies. We propose that the cardiac SP cell population functions as a progenitor cell population for the development, maintenance, and repair of the heart.

Original languageEnglish (US)
Pages (from-to)262-275
Number of pages14
JournalDevelopmental Biology
Volume265
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

Side-Population Cells
ATP-Binding Cassette Transporters
Stem Cells
Population
Embryonic Development
Maintenance
Yolk Sac
Embryonic Stem Cells
Cardiac Myocytes
Cell Differentiation
Cell Proliferation
Technology
Phenotype

Keywords

  • Abcg2
  • ATP-binding cassette transporter
  • Cardiac SP cells

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart. / Martin, Cindy M.; Meeson, Annette P.; Robertson, Scott M.; Hawke, Thomas J.; Richardson, James A.; Bates, Susan; Goetsch, Sean C.; Gallardo, Teresa D.; Garry, Daniel J.

In: Developmental Biology, Vol. 265, No. 1, 01.01.2004, p. 262-275.

Research output: Contribution to journalArticle

Martin, Cindy M. ; Meeson, Annette P. ; Robertson, Scott M. ; Hawke, Thomas J. ; Richardson, James A. ; Bates, Susan ; Goetsch, Sean C. ; Gallardo, Teresa D. ; Garry, Daniel J. / Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart. In: Developmental Biology. 2004 ; Vol. 265, No. 1. pp. 262-275.
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AU - Richardson, James A.

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