Pet-1 is required across different stages of life to regulate serotonergic function

Chen Liu, Takashi Maejima, Steven C. Wyler, Gemma Casadesus, Stefan Herlitze, Evan S. Deneris

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113 Scopus citations

Abstract

Transcriptional cascades are required for the specification of serotonin (5-HT) neurons and behaviors modulated by 5-HT. Several cascade factors are expressed throughout the lifespan, which suggests that their control of behavior might not be temporally restricted to programming normal numbers of 5-HT neurons. We used new mouse conditional targeting approaches to investigate the ongoing requirements for Pet-1 (also called Fev), a cascade factor that is required for the initiation of 5-HT synthesis, but whose expression persists into adulthood. We found that Pet-1 was required after the generation of 5-HT neurons for multiple steps in 5-HT neuron maturation, including axonal innervation of the somatosensory cortex, expression of appropriate firing properties, and the expression of the Htr1a and Htr1b autoreceptors. Pet-1 was still required in adult 5-HT neurons to preserve normal anxiety-related behaviors through direct autoregulated control of serotonergic gene expression. These findings indicate that Pet-1 is required across the lifespan of the mouse and that behavioral pathogenesis can result from both developmental and adult-onset alterations in serotonergic transcription.

Original languageEnglish (US)
Pages (from-to)1190-1198
Number of pages9
JournalNature neuroscience
Volume13
Issue number10
DOIs
StatePublished - Oct 1 2010

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Liu, C., Maejima, T., Wyler, S. C., Casadesus, G., Herlitze, S., & Deneris, E. S. (2010). Pet-1 is required across different stages of life to regulate serotonergic function. Nature neuroscience, 13(10), 1190-1198. https://doi.org/10.1038/nn.2623