PET imaging of medulloblastoma with an 18F-labeled tryptophan analogue in a transgenic mouse model

Yangchun Xin, Xuyi Yue, Hua Li, Zhiqin Li, Hancheng Cai, Arabinda K. Choudhary, Shaohui Zhang, Diane C. Chugani, Sigrid A. Langhans

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Abstract

In vivo positron emission tomography (PET) imaging is a key modality to evaluate disease status of brain tumors. In recent years, tremendous efforts have been made in developing PET imaging methods for pediatric brain tumors. Carbon-11 labelled tryptophan derivatives are feasible as PET imaging probes in brain tumor patients with activation of the kynurenine pathway, but the short half-life of carbon-11 limits its application. Using a transgenic mouse model for the sonic hedgehog (Shh) subgroup of medulloblastoma, here we evaluated the potential of the newly developed 1-(2-[18F]fluoroethyl)-L-tryptophan (1-L-[18F]FETrp) as a PET imaging probe for this common malignant pediatric brain tumor. 1-L-[18F]FETrp was synthesized on a PETCHEM automatic synthesizer with good chemical and radiochemical purities and enantiomeric excess values. Imaging was performed in tumor-bearing Smo/Smo medulloblastoma mice with constitutive actvation of the Smoothened (Smo) receptor using a PerkinElmer G4 PET-X-Ray scanner. Medulloblastoma showed significant and specific accumulation of 1-L-[18F]FETrp. 1-L-[18F]FETrp also showed significantly higher tumor uptake than its D-enantiomer, 1-D-[18F]FETrp. The uptake of 1-L-[18F]FETrp in the normal brain tissue was low, suggesting that 1-L-[18F]FETrp may prove a valuable PET imaging probe for the Shh subgroup of medulloblastoma and possibly other pediatric and adult brain tumors.

Original languageEnglish (US)
Article number3800
JournalScientific reports
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2020

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    Xin, Y., Yue, X., Li, H., Li, Z., Cai, H., Choudhary, A. K., Zhang, S., Chugani, D. C., & Langhans, S. A. (2020). PET imaging of medulloblastoma with an 18F-labeled tryptophan analogue in a transgenic mouse model. Scientific reports, 10(1), [3800]. https://doi.org/10.1038/s41598-020-60728-6