Pet10p is a yeast perilipin that stabilizes lipid droplets and promotes their assembly

Qiang Gao, Derk D. Binns, Lisa N. Kinch, Nick V. Grishin, Natalie Ortiz, Xiao Chen, Joel M. Goodman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Pet10p is a yeast lipid droplet protein of unknown function. We show that it binds specifically to and is stabilized by droplets containing triacylglycerol (TG). Droplets isolated from cells with a PET10 deletion strongly aggregate, appear fragile, and fuse in vivo when cells are cultured in oleic acid. Pet10p binds early to nascent droplets, and their rate of appearance is decreased in pet10Δ. Moreover, Pet10p functionally interacts with the endoplasmic reticulum droplet assembly factors seipin and Fit2 to maintain proper droplet morphology. The activity of Dga1p, a diacylglycerol acyltransferase, and TG accumulation were both 30-35% lower in the absence of Pet10p. Pet10p contains a PAT domain, a defining property of perilipins, which was not previously known to exist in yeast. We propose that the core functions of Pet10p and other perilipins extend beyond protection from lipases and include the preservation of droplet integrity as well as collaboration with seipin and Fit2 in droplet assembly and maintenance.

Original languageEnglish (US)
Pages (from-to)3199-3217
Number of pages19
JournalJournal of Cell Biology
Volume216
Issue number10
DOIs
StatePublished - Oct 1 2017

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Triglycerides
Yeasts
Diacylglycerol O-Acyltransferase
Fungal Proteins
Oleic Acid
Lipase
Endoplasmic Reticulum
Cultured Cells
Maintenance
Lipid Droplets
Perilipin-1
Perilipins
Lipid Droplet Associated Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Pet10p is a yeast perilipin that stabilizes lipid droplets and promotes their assembly. / Gao, Qiang; Binns, Derk D.; Kinch, Lisa N.; Grishin, Nick V.; Ortiz, Natalie; Chen, Xiao; Goodman, Joel M.

In: Journal of Cell Biology, Vol. 216, No. 10, 01.10.2017, p. 3199-3217.

Research output: Contribution to journalArticle

Gao, Qiang ; Binns, Derk D. ; Kinch, Lisa N. ; Grishin, Nick V. ; Ortiz, Natalie ; Chen, Xiao ; Goodman, Joel M. / Pet10p is a yeast perilipin that stabilizes lipid droplets and promotes their assembly. In: Journal of Cell Biology. 2017 ; Vol. 216, No. 10. pp. 3199-3217.
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