PG13 packaging cells produce recombinant retroviruses carrying a diphtheria toxin mutant which kills cancer cells.

Jian Qiao, Manuel Caruso

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The development of suicide gene therapy with gene products that are directly toxic to cells, such as the A subunit of diphtheria toxin (DT-A), has been hampered by the difficulty of engineering recombinant viruses. DT-A is a strong inhibitor of protein synthesis that acts by ADP-ribosylating elongation factor 2, and a low level of DT-A expression in virus producer cells prevents the production of recombinant virus. We analyzed here the natural resistance of packaging cells to DT-A toxicity, and we report that PG13 and PA317 packaging cell lines are resistant to H21G, a DT-A mutant. PG13 cells produce recombinant H21G virus that efficiently kills a variety of human tumor cells. Our finding indicates that PG13 packaging cells provide a new potential for the development of DT-A-based suicide gene therapy.

Original languageEnglish (US)
Pages (from-to)7343-7348
Number of pages6
JournalJournal of virology
Volume76
Issue number14
DOIs
StatePublished - Jul 2002

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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