PG13 packaging cells produce recombinant retroviruses carrying a diphtheria toxin mutant which kills cancer cells.

Jian Qiao, Manuel Caruso

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The development of suicide gene therapy with gene products that are directly toxic to cells, such as the A subunit of diphtheria toxin (DT-A), has been hampered by the difficulty of engineering recombinant viruses. DT-A is a strong inhibitor of protein synthesis that acts by ADP-ribosylating elongation factor 2, and a low level of DT-A expression in virus producer cells prevents the production of recombinant virus. We analyzed here the natural resistance of packaging cells to DT-A toxicity, and we report that PG13 and PA317 packaging cell lines are resistant to H21G, a DT-A mutant. PG13 cells produce recombinant H21G virus that efficiently kills a variety of human tumor cells. Our finding indicates that PG13 packaging cells provide a new potential for the development of DT-A-based suicide gene therapy.

Original languageEnglish (US)
Pages (from-to)7343-7348
Number of pages6
JournalJournal of Virology
Volume76
Issue number14
DOIs
StatePublished - Jan 1 2002

Fingerprint

Retroviridae
Diphtheria Toxin
Product Packaging
packaging
toxins
mutants
suicide genes
Viruses
Neoplasms
viruses
gene therapy
cells
Genetic Therapy
Suicide
Peptide Elongation Factor 2
protein synthesis inhibitors
Protein Synthesis Inhibitors
Poisons
Innate Immunity
Adenosine Diphosphate

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

PG13 packaging cells produce recombinant retroviruses carrying a diphtheria toxin mutant which kills cancer cells. / Qiao, Jian; Caruso, Manuel.

In: Journal of Virology, Vol. 76, No. 14, 01.01.2002, p. 7343-7348.

Research output: Contribution to journalArticle

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