PGE from Octopus aegina induces apoptosis in Ehrlich's ascites carcinoma of mice

S. Karthigayan, M. Sri Balasubashini, T. Balasubramanian, S. T. Somasundaram

Research output: Contribution to journalArticle

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Abstract

The present study was carried out to assess the antitumor effect of venomous peptide from the cephalopod Octopus aegina on Ehrlich's ascites carcinoma (EAC). Male albino Swiss mice were used in the present study. Four groups of animals were treated with three doses of the sublethal dose of venom, 15, 75, and 150 μg/kg body weight (intraperitoneal injection), along with the standard drug 5-fluorouracil (20 mg/kg b.w.). After 10 days of treatment, six animals from each group were sacrificed for the biochemical analysis and the rest were left to calculate the mean survival time. In EAC-bearing mice, mean lifespan, tumor volume, hemoglobin, red blood cells, and lymphocytes were significantly decreased when compared to the normal animals. While body weight, neutrophils, and viable tumor cell count were increased in the EAC-bearing mice, these changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as DNA, RNA, and protein, were altered in the EAC-bearing mice and observed to be near normal in the treatment groups. The caspase-3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC-bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, octopus venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Therefore, the venom from O. aegina has a potential antitumor effect on the EAC-bearing mice.

Original languageEnglish (US)
Pages (from-to)451-458
Number of pages8
JournalToxicology Mechanisms and Methods
Volume17
Issue number8
DOIs
StatePublished - Oct 1 2007

Fingerprint

Bearings (structural)
Octopodiformes
Prostaglandins E
Ascites
Apoptosis
Carcinoma
Tumors
Animals
Cells
Venoms
Mollusk Venoms
Tumor Burden
Lymphocytes
DNA
Cell death
Body Weight
Aspartate Aminotransferases
Cephalopoda
Alanine Transaminase
Macromolecules

Keywords

  • Caspase-3
  • DNA Fragmentation
  • EAC
  • Octopus Venom

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

PGE from Octopus aegina induces apoptosis in Ehrlich's ascites carcinoma of mice. / Karthigayan, S.; Balasubashini, M. Sri; Balasubramanian, T.; Somasundaram, S. T.

In: Toxicology Mechanisms and Methods, Vol. 17, No. 8, 01.10.2007, p. 451-458.

Research output: Contribution to journalArticle

Karthigayan, S. ; Balasubashini, M. Sri ; Balasubramanian, T. ; Somasundaram, S. T. / PGE from Octopus aegina induces apoptosis in Ehrlich's ascites carcinoma of mice. In: Toxicology Mechanisms and Methods. 2007 ; Vol. 17, No. 8. pp. 451-458.
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