pH-dependence of interaction between melittin and baeteriorhodopsin

Qiuxing Jiang, Kunsheng Hu, Wei Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Melittin differentially slowed down the fast (M412f) and the slow (M412s) decay components of the photocycle intermediate M of trimeric bacteriorhodopsin in purple membrane while it accelerated the M412s of Triton X-100-solubilized bacteriorhodopsin monomers. Raising the bulk pH could enhance the effect of melittin on the M412s of bacteriorhodopsin in these two states. From pH 5.5 to 8.8, melittin slightly influenced the yield of intermediate M in purple membrane, whereas the yield of M412s decreased and subsequently reversed with the addition of melittin. Moreover, the monomeric bacteriorhodopsin bleached more readily in the presence of melittin and the higher pH made the bleaching effect of melittin more intensive as well. These results re-certify our former suggestions that there was electrostatic interaction between melittin and bacteriorhodopsin, and indicate that the biphasic M decay may not result from the well-known linear kinetic scheme (M → N → BR). At last the mechanisms underlying the interaction of melittin with purple membranes and bacteriorhodopsin monomers are analyzed.

Original languageEnglish (US)
Pages (from-to)362-372
Number of pages11
JournalScience in China, Series C: Life Sciences
Volume39
Issue number4
StatePublished - Aug 1996

Fingerprint

melittin
Melitten
Bacteriorhodopsins
membrane
Purple Membrane
bleaching
Membranes
kinetics
Monomers
deterioration
electrostatic interactions
Octoxynol
Bleaching
Coulomb interactions
Static Electricity
bacteriorhodopsins
effect

Keywords

  • Bacteriorhodopsin
  • Electrostatic interaction
  • Melittin
  • Photocycle

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

pH-dependence of interaction between melittin and baeteriorhodopsin. / Jiang, Qiuxing; Hu, Kunsheng; Wang, Wei.

In: Science in China, Series C: Life Sciences, Vol. 39, No. 4, 08.1996, p. 362-372.

Research output: Contribution to journalArticle

Jiang, Qiuxing ; Hu, Kunsheng ; Wang, Wei. / pH-dependence of interaction between melittin and baeteriorhodopsin. In: Science in China, Series C: Life Sciences. 1996 ; Vol. 39, No. 4. pp. 362-372.
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