Ph tolerance of rabbit corneal endothelium in tissue culture

Dale R. Meyer, James P McCulley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The pH tolerance of rabbit corneal endothelial cultures was determined for a balanced salt solution and various pharmaceutical vehicles in an effort to determine the biocompatibility of the different formulations after 4-128 min exposures. Acute damage was assessed by monitoring the release of radiolabeled nucleotides and corresponding changes in tissue morphology of the prelabeled cultures. Protracted damage was evaluated by re-examining the morphology of the same cultures after 24 hr along with quantitative measurement of 3H release, tissue loss, and concomitant changes in protein synthesis. Our testing results indicated that the permissive limits of a balanced salt solution were between a pH of 6 and 8, which is comparable to the guidelines for native endothelium in situ established by organ perfusion studies. In contrast, the permissive limits for prototypes of intraocular vehicles were frequently more restricted, since only unbuffered solutions at a neutral pH were relatively nontoxic. Topical vehicles, by comparison, were much more damaging, which generally contraindicates their intraocular use. The differential sensitivity of the tissue confirmed our suspicions that pH is a more critical factor in these types of formulations. These data indicate that this type of bioassay can be used effectively for screening in drug development programs, and for establishing optimal guidelines for pharmaceutical formulations.

Original languageEnglish (US)
Pages (from-to)15-30
Number of pages16
JournalCutaneous and Ocular Toxicology
Volume11
Issue number1
DOIs
StatePublished - 1992

Fingerprint

Corneal Endothelium
Tissue culture
Tissue
Rabbits
Pharmaceutical Vehicles
Salts
Bioassay
Guidelines
Biocompatibility
Cell culture
Pharmaceutical Preparations
Drug Compounding
Preclinical Drug Evaluations
Screening
Nucleotides
Biological Assay
Endothelium
Perfusion
Monitoring
Testing

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Ophthalmology

Cite this

Ph tolerance of rabbit corneal endothelium in tissue culture. / Meyer, Dale R.; McCulley, James P.

In: Cutaneous and Ocular Toxicology, Vol. 11, No. 1, 1992, p. 15-30.

Research output: Contribution to journalArticle

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