Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration

Christopher A. Lopez, Sebastian E. Winter, Fabian Rivera-Chávez, Mariana N. Xavier, Victor Poon, Sean Paul Nuccio, Renée M. Tsolis, Andreas J. Bäumler

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Information on how emerging pathogens can invade and persist and spread within host populations remains sparse. In the 1980s, a multidrug-resistant Salmonella enterica serotype Typhimurium clone lysogenized by a bacteriophage carrying the sopE virulence gene caused an epidemic among cattle and humans in Europe. Here we show that phage-mediated horizontal transfer of the sopE gene enhances the production of host-derived nitrate, an energetically highly valuable electron acceptor, in a mouse colitis model. In turn, nitrate fuels a bloom of S. Typhimurium in the gut lumen through anaerobic nitrate respiration while suppressing genes for the utilization of energetically inferior electron acceptors such as tetrathionate. Through this mechanism, horizontal transfer of sopE can enhance the fitness of S. Typhimurium, resulting in its significantly increased abundance in the feces.

Original languageEnglish (US)
JournalmBio
Volume3
Issue number3
DOIs
StatePublished - May 2012

Fingerprint

Salmonella
Nitrates
Bacteriophages
Respiration
Growth
Electrons
Horizontal Gene Transfer
Proteins
Salmonella enterica
Colitis
Feces
Genes
Virulence
Clone Cells
Population
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration. / Lopez, Christopher A.; Winter, Sebastian E.; Rivera-Chávez, Fabian; Xavier, Mariana N.; Poon, Victor; Nuccio, Sean Paul; Tsolis, Renée M.; Bäumler, Andreas J.

In: mBio, Vol. 3, No. 3, 05.2012.

Research output: Contribution to journalArticle

Lopez, CA, Winter, SE, Rivera-Chávez, F, Xavier, MN, Poon, V, Nuccio, SP, Tsolis, RM & Bäumler, AJ 2012, 'Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration', mBio, vol. 3, no. 3. https://doi.org/10.1128/mBio.00143-12
Lopez, Christopher A. ; Winter, Sebastian E. ; Rivera-Chávez, Fabian ; Xavier, Mariana N. ; Poon, Victor ; Nuccio, Sean Paul ; Tsolis, Renée M. ; Bäumler, Andreas J. / Phage-mediated acquisition of a type III secreted effector protein boosts growth of salmonella by nitrate respiration. In: mBio. 2012 ; Vol. 3, No. 3.
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