Phase shifting the circadian clock with cycloheximide: response of hamster with an intact or a split rhythm of locomotor activity

Franziska Wollnik, Fred W. Turek, Philip Majewski, Joseph S. Takahashi

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Systematic administration of the protein synthesis inhibitor, cycloheximide, induced both phase advances and phase delays in the circadian rhythm of wheel-running activity in hamsters (Mesocricetus auratus) maintained in constant darkness or constant light. The magnitude and direction of the phase shifts were dependent on the circadian time (CT) of drug treatment. The phase response curves in constant darkness and constant light were of similar general shape, but they differed in the overall mean amplitude of the phase shifts. Maximal phase advances were observed after injections around CT 6-8, maximal delays at CT 0-2. Injections of various doses of cycloheximide at CT 0 induced a dose-dependent phase delay in the rhythm with a maximum delay induced by 10 mg cycloheximide. Injections of cycloheximide in animals with a split activity rhythm caused phase shifts of both components in the same direction (20/39) and in different directions (10/39). The results support the hypothesis that 80S ribosomal protein synthesis plays an important role in the biochemical mechanisms of circadian systems.

Original languageEnglish (US)
Pages (from-to)82-88
Number of pages7
JournalBrain Research
Volume496
Issue number1-2
DOIs
StatePublished - Sep 4 1989

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Keywords

  • Circadian rhythm
  • Cycloheximide
  • Hamster
  • Phase response curve
  • Protein synthesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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