Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells

Kathryn E. Kistler, Tatjana Trcek, Thomas R. Hurd, Ruoyu Chen, Feng Xia Liang, Joseph Sall, Masato Kato, Ruth Lehmann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Germ granules are non-membranous ribonucleoprotein granules deemed the hubs for post-transcriptional gene regulation and functionally linked to germ cell fate across species. Little is known about the physical properties of germ granules and how these relate to germ cell function. Here we study two types of germ granules in the Drosophila embryo: cytoplasmic germ granules that instruct primordial germ cells (PGCs) formation and nuclear germ granules within early PGCs with unknown function. We show that cytoplasmic and nuclear germ granules are phase transitioned condensates nucleated by Oskar protein that display liquid as well as hydrogel-like properties. Focusing on nuclear granules, we find that Oskar drives their formation in heterologous cell systems. Multiple, independent Oskar protein domains synergize to promote granule phase separation. Deletion of Oskar’s nuclear localization sequence specifically ablates nuclear granules in cell systems. In the embryo, nuclear germ granules promote germ cell divisions thereby increasing PGC number for the next generation.

Original languageEnglish (US)
Article numbere37949
JournaleLife
Volume7
DOIs
StatePublished - Sep 1 2018

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Germ Cells
Cell Division
Drosophila
Cells
Ribonucleoproteins
Hydrogel
Embryonic Structures
Gene expression
Phase separation
Cytoplasmic Granules
Proteins
Physical properties
Liquids
Cell Count
Genes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kistler, K. E., Trcek, T., Hurd, T. R., Chen, R., Liang, F. X., Sall, J., ... Lehmann, R. (2018). Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells. eLife, 7, [e37949]. https://doi.org/10.7554/eLife.37949

Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells. / Kistler, Kathryn E.; Trcek, Tatjana; Hurd, Thomas R.; Chen, Ruoyu; Liang, Feng Xia; Sall, Joseph; Kato, Masato; Lehmann, Ruth.

In: eLife, Vol. 7, e37949, 01.09.2018.

Research output: Contribution to journalArticle

Kistler, KE, Trcek, T, Hurd, TR, Chen, R, Liang, FX, Sall, J, Kato, M & Lehmann, R 2018, 'Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells', eLife, vol. 7, e37949. https://doi.org/10.7554/eLife.37949
Kistler KE, Trcek T, Hurd TR, Chen R, Liang FX, Sall J et al. Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells. eLife. 2018 Sep 1;7. e37949. https://doi.org/10.7554/eLife.37949
Kistler, Kathryn E. ; Trcek, Tatjana ; Hurd, Thomas R. ; Chen, Ruoyu ; Liang, Feng Xia ; Sall, Joseph ; Kato, Masato ; Lehmann, Ruth. / Phase transitioned nuclear oskar promotes cell division of drosophila primordial germ cells. In: eLife. 2018 ; Vol. 7.
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