Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated from patients with rheumatoid arthritis

John J. Cush, Peter E. Lipsky

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Abstract

Cytofluorometric analysis was performed to characterize the surface phenotype and activation status of freshly isolated synovial tissue lymphocytes (STL) and peripheral blood lymphocytes (PBL) from 7 patients with rheumatoid arthritis (RA). Proliferative synovium was enzymatically digested to obtain tissue‐derived lymphocytes. Indirect immunofluorescent staining of patient PBL and STL with a variety of monoclonal antibodies failed to reveal a consistent alteration in the number of CD4+ (helper/inducer) PBL or STL. However, there was a significant decrease in the number of CD8+ (suppressor/cytotoxic) cells in rheumatoid STL (P < 0.05). A significant reduction in the density of the T cell differentiation antigens CD3 and CD4 was observed in RA PBL and STL, compared with control PBL. These differences in antigen density were not seen when normal PBL were subjected to the same enzymatic digestion. Both RA PBL and STL manifested increased expression of HLA–DR antigens, without augmentation of interleukin‐2 receptor expression. Alterations in the expression of the T cell differentiation antigens and activation antigens by patient PBL closely paralleled the abnormalities observed in STL. In contrast, STL of patients with RA exhibited an increase in the expression of the adhesion‐related glycoproteins (leukocyte function‐associated 1 [LFA‐1] and very late activation 1 [VLA‐1] antigens), not observed with autologous PBL. These studies demonstrate that lymphocytes isolated from the synovial tissues of RA patients bear an activated phenotype, exemplified by the modulation of CD3 and CD4 and the expression of HLA‐DR. The enhanced expression of the adhesion molecules LFA‐1 and VLA‐1 by STL may play a role in the localization of these cells to the inflamed synovium.

Original languageEnglish (US)
Pages (from-to)1230-1238
Number of pages9
JournalArthritis & Rheumatism
Volume31
Issue number10
DOIs
StatePublished - Oct 1988

Fingerprint

Rheumatoid Arthritis
Lymphocytes
T Lymphocyte Differentiation Antigens
Antigens
Synovial Membrane
Phenotype
Cell Adhesion Molecules
Digestion

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Phenotypic analysis of synovial tissue and peripheral blood lymphocytes isolated from patients with rheumatoid arthritis. / Cush, John J.; Lipsky, Peter E.

In: Arthritis & Rheumatism, Vol. 31, No. 10, 10.1988, p. 1230-1238.

Research output: Contribution to journalArticle

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