Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells

B. F. Haynes, R. S. Metzgar, J. D. Minna, P. A. Bunn

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Abstract

We studied the surface-antigen pattern of T cells in peripheral blood and cell lines from patients with advanced cutaneous T-cell lymphoma (CTCL) or T-cell acute lymphoblastic leukemia. The antigen patterns of cutaneous T-cell lymphoma cells from peripheral blood and established cell lines were nearly identical; the cells were negative for human thymus antigen (OKT6 and NA1/34), positive for pan-T-cell (OKT3, 17F12, 10.2, and 9.6) and helper-T-cell-subset (OKT4) antigens, and negative for T-cell-subset antigens 3A1 and OKT8. In contrast, the phenotypes of malignant T cells from patients with acute lymphoblastic leukemia were heterogeneous, with at least five patterns of reactivity. The T-cell-specific antibody 3A1 was the only monoclonal reagent that clearly distinguished the peripheral-blood T cells in CTCL (3A1-) from those in acute lymphoblastic leukemia (3A1+). More over, 3A1 was the most reliable T-cell marker in acute lymphoblastic leukemia. We conclude that circulating CTCL (Sezary) T cells are homogeneous in their antigen phenotype and are derived from a well-differentiated 3A1-, OKT4+, OKT8- helper-T-cell subset.

Original languageEnglish (US)
Pages (from-to)1319-1323
Number of pages5
JournalNew England Journal of Medicine
Volume304
Issue number22
StatePublished - 1981

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Cutaneous T-Cell Lymphoma
Monoclonal Antibodies
T-Lymphocytes
Antigens
T-Lymphocyte Subsets
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Blood Cells
Helper-Inducer T-Lymphocytes
Phenotype
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Muromonab-CD3
Cell Line
Surface Antigens
Thymus Gland
Antibodies

ASJC Scopus subject areas

  • Medicine(all)

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Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells. / Haynes, B. F.; Metzgar, R. S.; Minna, J. D.; Bunn, P. A.

In: New England Journal of Medicine, Vol. 304, No. 22, 1981, p. 1319-1323.

Research output: Contribution to journalArticle

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