Phenotypic complementation of genetic immunodeficiency by chronic herpesvirus infection

Donna A. MacDuff, Tiffany A. Reese, Jacqueline M. Kimmey, Leslie A. Weiss, Christina Song, Xin Zhang, Amal Kambal, Erning Duan, Javier A. Carrero, Bertrand Boisson, Emmanuel Laplantine, Alain Israel, Capucine Picard, Marco Colonna, Brian T. Edelson, L. David Sibley, Christina L. Stallings, Jean Laurent Casanova, Kazuhiro Iwai, Herbert W. Virgin

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Variation in the presentation of hereditary immunodeficiencies may be explained by genetic or environmental factors. Patients with mutations in HOIL1 (RBCK1) present with amylopectinosis-associated myopathy with or without hyper-inflammation and immunodeficiency. We report that barrier-raised HOIL-1-deficient mice exhibit amylopectin-like deposits in the myocardium but show minimal signs of hyper-inflammation. However, they show immunodeficiency upon acute infection with Listeria monocytogenes, Toxoplasma gondii or Citrobacter rodentium. Increased susceptibility to Listeria was due to HOIL-1 function in hematopoietic cells and macrophages in production of protective cytokines. In contrast, HOIL-1-deficient mice showed enhanced control of chronic Mycobacterium tuberculosis or murine γ-herpesvirus 68 (MHV68), and these infections conferred a hyper-inflammatory phenotype. Surprisingly, chronic infection with MHV68 complemented the immunodeficiency of HOIL-1, IL-6, Caspase-1 and Caspase-1;Caspase-11-deficient mice following Listeria infection. Thus chronic herpesvirus infection generates signs of auto-inflammation and complements genetic immunodeficiency in mutant mice, highlighting the importance of accounting for the virome in genotype-phenotype studies.

Original languageEnglish (US)
Article numbere04494
JournaleLife
Volume2015
Issue number4
DOIs
StatePublished - Jan 20 2015

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    MacDuff, D. A., Reese, T. A., Kimmey, J. M., Weiss, L. A., Song, C., Zhang, X., Kambal, A., Duan, E., Carrero, J. A., Boisson, B., Laplantine, E., Israel, A., Picard, C., Colonna, M., Edelson, B. T., Sibley, L. D., Stallings, C. L., Casanova, J. L., Iwai, K., & Virgin, H. W. (2015). Phenotypic complementation of genetic immunodeficiency by chronic herpesvirus infection. eLife, 2015(4), [e04494]. https://doi.org/10.7554/eLife.04494