Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e

Christoph Aebi, Eric R. Lafontaine, Leslie D. Cope, Jo L. Latimer, Sheryl L. Lumbley, George H. Mccracken, Eric J. Hansen

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Abstract

The UspA surface antigen of Moraxella catarrhalis was recently shown to be comprised of two different proteins (UspA1 and UspA2) which share an internal region containing 140 amino acids with 93% identity (C. Aebi, I. Maciver, J. L. Latimer, L. D. Cope, M. K. Stevens, S. E. Thomas, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 65:4367-4377, 1997). Isogenic uspA1, uspA2, and uspA1 uspA2 mutants were tested in a number of in vitro systems to determine what effect these mutations, either individually or together, might exert on the phenotype of M. catarrhalis 035E. Monoclonal antibodies specific for UspA1 or UspA2 were used in an indirect antibody accessibility assay to prove that both of these proteins were expressed on the surface of M. catarrhalis. All three mutants grew in vitro at the same rate and did not exhibit autoagglutination or hemagglutination properties that were detectably different from those of the wild-type parent strain. When tested for the ability to adhere to human epithelial cells, the wild- type parent strain and the uspA2 mutant readily attached to Chang conjunctival cells. In contrast, the uspA1 mutant and the uspA1 uspA2 double mutant both attached to these epithelial cells at a level nearly 2 orders of magnitude lower than that obtained with the wildtype parent strain, a result which suggested that expression of UspA1 by M. catarrhalis is essential for attachment to these epithelial cells. Both the wild-type parent strain and the uspA1 mutant were resistant to the bactericidal activity of normal human serum, whereas the uspA2 mutant and the uspA1 uspA2 double mutant were readily killed by this serum. This latter result indicated that the presence of UspA2 is essential for expression of serum resistance by M. catarrhalis.

Original languageEnglish (US)
Pages (from-to)3113-3119
Number of pages7
JournalInfection and Immunity
Volume66
Issue number7
StatePublished - Jul 1998

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Moraxella (Branhamella) catarrhalis
Mutation
Epithelial Cells
Serum
Hemagglutination
Surface Antigens
Human Activities
Proteins
Monoclonal Antibodies
Phenotype
Amino Acids
Antibodies
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

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Aebi, C., Lafontaine, E. R., Cope, L. D., Latimer, J. L., Lumbley, S. L., Mccracken, G. H., & Hansen, E. J. (1998). Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e. Infection and Immunity, 66(7), 3113-3119.

Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e. / Aebi, Christoph; Lafontaine, Eric R.; Cope, Leslie D.; Latimer, Jo L.; Lumbley, Sheryl L.; Mccracken, George H.; Hansen, Eric J.

In: Infection and Immunity, Vol. 66, No. 7, 07.1998, p. 3113-3119.

Research output: Contribution to journalArticle

Aebi, C, Lafontaine, ER, Cope, LD, Latimer, JL, Lumbley, SL, Mccracken, GH & Hansen, EJ 1998, 'Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e', Infection and Immunity, vol. 66, no. 7, pp. 3113-3119.
Aebi C, Lafontaine ER, Cope LD, Latimer JL, Lumbley SL, Mccracken GH et al. Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e. Infection and Immunity. 1998 Jul;66(7):3113-3119.
Aebi, Christoph ; Lafontaine, Eric R. ; Cope, Leslie D. ; Latimer, Jo L. ; Lumbley, Sheryl L. ; Mccracken, George H. ; Hansen, Eric J. / Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035e. In: Infection and Immunity. 1998 ; Vol. 66, No. 7. pp. 3113-3119.
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