Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells

J. L. Nixon-Fulton, J. Hackett, P. R. Bergstresser, V. Kumar, R. E. Tigelaar

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Abstract

Short-term and long-term cultures of mouse Thy-1+ epidermal cells (EC) were established in order to characterize their phenotypic and functional properties. Concanavalin A (Con A) and Interleukin 2 (IL-2) stimulated Thy-1+ EC mediated non-MHC directed cytotoxicity preferentially against the NK-sensitive target, YAC-1 vs the NK-resistant target, P815; these cells also mediated antibody-dependent cell-mediated cytotoxicity (ADCC), indicating the presence of IgG-FcR on at least some of them. Freshly isolated Thy-1+ EC failed to lyse YAC-1 targets; however, this activity was observed after 9 d of culture with Con A and IL-2. While dendritic Thy-1+ EC, in vivo, do not express the T-cell markers, L3T4 and Lyt-2, short-term cultured cells displayed phenotypic heterogeneity with small but significant percentages of Lyt-2+ and L3T4+ cells appearing transiently. The phenotype of the effector cell(s), which mediates cytotoxic activity, was determined by utilizing flow cytometry to sort short-term cultured EC into positively and negatively stained populations. Cells which express L3T4, or which lack asialo GM1, did not lyse YAC-1 targets; maximum cytotoxic activity was found within populations of cells which are asialo GM1 +, Lyt-2-, and asialo GM1 +, Lyt-2+. These studies indicate that Thy-1+ cells derived from mouse epidermis when cultured in the presence of Con A and IL-2 have the capacity to generate a phenotypically heterogeneous population, some cells of which are capable of mediating cytotoxic activities.

Original languageEnglish (US)
Pages (from-to)62-68
Number of pages7
JournalJournal of Investigative Dermatology
Volume91
Issue number1
StatePublished - 1988

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Concanavalin A
Interleukin-2
Cytotoxicity
Cells
T-cells
Flow cytometry
Immunoglobulin G
Antibodies
Cultured Cells
asialo GM1 ganglioside
Population
Antibody-Dependent Cell Cytotoxicity
Epidermis
Flow Cytometry
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Dermatology

Cite this

Nixon-Fulton, J. L., Hackett, J., Bergstresser, P. R., Kumar, V., & Tigelaar, R. E. (1988). Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells. Journal of Investigative Dermatology, 91(1), 62-68.

Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells. / Nixon-Fulton, J. L.; Hackett, J.; Bergstresser, P. R.; Kumar, V.; Tigelaar, R. E.

In: Journal of Investigative Dermatology, Vol. 91, No. 1, 1988, p. 62-68.

Research output: Contribution to journalArticle

Nixon-Fulton, JL, Hackett, J, Bergstresser, PR, Kumar, V & Tigelaar, RE 1988, 'Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells', Journal of Investigative Dermatology, vol. 91, no. 1, pp. 62-68.
Nixon-Fulton, J. L. ; Hackett, J. ; Bergstresser, P. R. ; Kumar, V. ; Tigelaar, R. E. / Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells. In: Journal of Investigative Dermatology. 1988 ; Vol. 91, No. 1. pp. 62-68.
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