Phosphatase PP2A and microtubule-mediated pulling forces disassemble centrosomes during mitotic exit

Stephen J. Enos, Martin Dressler, Beatriz Ferreira Gomes, Anthony A. Hyman, Jeffrey B. Woodruff

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Centrosomes are microtubule-nucleating organelles that facilitate chromosome segregation and cell division in metazoans. Centrosomes comprise centrioles that organize a micron-scale mass of protein called pericentriolar material (PCM) from which microtubules nucleate. During each cell cycle, PCM accumulates around centrioles through phosphorylation-mediated assembly of PCM scaffold proteins. During mitotic exit, PCM swiftly disassembles by an unknown mechanism. Here, we used Caenorhabditis elegans embryos to determine the mechanism and importance of PCM disassembly in dividing cells. We found that the phosphatase PP2A and its regulatory subunit SUR-6 (PP2ASUR-6), together with cortically directed microtubule pulling forces, actively disassemble PCM. In embryos depleted of these activities, ∼25% of PCM persisted from one cell cycle into the next. Purified PP2ASUR-6 could dephosphorylate the major PCM scaffold protein SPD-5 in vitro. Our data suggest that PCM disassembly occurs through a combination of dephosphorylation of PCM components and force-driven fragmentation of the PCM scaffold.

Original languageEnglish (US)
Article number029777
JournalBiology Open
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

centrosomes
Centrosome
Phosphoric Monoester Hydrolases
Microtubules
microtubules
Centrioles
Cell Cycle
Embryonic Structures
Chromosome Segregation
Proteins
Caenorhabditis elegans
Organelles
Cell Division
Scaffolds
scaffolding proteins
Phosphorylation
centrioles
Cells
cell cycle
chromosome segregation

Keywords

  • Centrosome
  • Disassembly
  • Let-92
  • Pericentriolar material
  • Phosphatase pp2a
  • Spd-5

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Phosphatase PP2A and microtubule-mediated pulling forces disassemble centrosomes during mitotic exit. / Enos, Stephen J.; Dressler, Martin; Gomes, Beatriz Ferreira; Hyman, Anthony A.; Woodruff, Jeffrey B.

In: Biology Open, Vol. 7, No. 1, 029777, 01.01.2018.

Research output: Contribution to journalArticle

Enos, Stephen J. ; Dressler, Martin ; Gomes, Beatriz Ferreira ; Hyman, Anthony A. ; Woodruff, Jeffrey B. / Phosphatase PP2A and microtubule-mediated pulling forces disassemble centrosomes during mitotic exit. In: Biology Open. 2018 ; Vol. 7, No. 1.
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