Phosphatidic acid phosphohydrolase and phospholipids in tracheal and amniotic fluids during normal ovine pregnancy

C. R. Rosenfeld, O. Andujo, J. M. Johnston, J. M. Jimenez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Samples of amniotic fluid and fetal tracheal fluid were obtained from 36 ovine pregnancies which were studied either acutely or chronically during the last two-thirds of gestation (65 to 149 days). The specific activity of phosphatidic acid phosphohydrolase (PAPase), disaturated lecithin (DS-L), total phospholipids (TPL), and the L/S ratio were assayed in the amniotic and tracheal fluids. There was a significant and progressive increase in the specific activity of PAPase in tracheal fluid beginning after 110 days, increasing from 66 ± 7 nmoles phosphate released x mg-1 protein x hour-1 (Mean ± S.E.M.) ≤ 110 days, to 107 ± 6 at 111 to 120 days, and to 277 ± 70 at 131 to 144 days. The rise in PAPase specific activity was followed by a parallel rise in the DS-L fraction of the TPL (DS-L/TPL ratio), increasing from a DS-L/TPL ratio of 0.06 ± 0.01 in fetuses ≤ 120 days, to 0.29 ± 0.06 at 121 to 130 days, and to 0.50 ± 0.18 at 131 ± 135 days. The PAPase specific activity and the L/S ratio in amniotic fluid did not change during pregnancy.

Original languageEnglish (US)
Pages (from-to)891-893
Number of pages3
JournalPediatric Research
Volume14
Issue number7
StatePublished - 1980

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Phosphatidate Phosphatase
Amniotic Fluid
Phospholipids
Sheep
Pregnancy
Fetus
Phosphates
disaturated lecithin
Proteins

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Phosphatidic acid phosphohydrolase and phospholipids in tracheal and amniotic fluids during normal ovine pregnancy. / Rosenfeld, C. R.; Andujo, O.; Johnston, J. M.; Jimenez, J. M.

In: Pediatric Research, Vol. 14, No. 7, 1980, p. 891-893.

Research output: Contribution to journalArticle

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