Phosphatidylinositol 3-kinase contributes to cell volume regulation through effects on ATP release

Andrew P. Feranchak, Richard M. Roman, Erik M. Schwiebert, J. Gregory Fitz

Research output: Contribution to journalArticle

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Abstract

Regulated changes in cell volume represent a signal that modulates a broad range of cell and organ functions. In HTC hepatoma cells, increases in volume are coupled to membrane ion permeability through a pathway involving (i) ATP efflux, (ii) autocrine stimulation of P2 receptors, and (iii) increases in anion permeability and Cl- efflux, contributing to recovery of volume toward basal values. Based on recent evidence that cell volume increases also stimulate phosphoinositide kinases, the purpose of these studies was to determine if phosphatidylinositol 3-kinase (PI 3-kinase) modulates these pathways. Exposure of cells to hypotonic buffer (20 or 40% less NaCl) caused an initial increase in cell volume and stimulated a rapid increase in ATP release. Subsequent opening of Cl- channels was followed by recovery of cell volume toward basal values, despite the continuous presence of hypotonic buffer. Inhibition of PI 3-kinase with wortmannin (K(i) = 3 nM) significantly inhibited both the rate of volume recovery and activation of Cl- currents; similar results were obtained with LY294002 (10 μM). Additionally, current activation was inhibited by intracellular dialysis with antibodies specific for the 110-kDa catalytic subunit of PI 3-kinase. Since release of ATP is a critical element in the volume-regulatory pathway, the role of PI 3-kinase on volume-stimulated ATP release was assessed. Both wortmannin and LY294002 decreased basal and volume-stimulated ATP permeability but had no effect on the current response to exogenous ATP (10 μM). These findings indicate that PI 3-kinase plays a significant role in regulation of cell volume and suggest that the effects are mediated in part through modulation of cellular ATP release.

Original languageEnglish (US)
Pages (from-to)14906-14911
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number24
DOIs
StatePublished - Jun 12 1998

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Phosphatidylinositol 3-Kinase
Cell Size
Adenosine Triphosphate
Permeability
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Recovery
Buffers
Chemical activation
1-Phosphatidylinositol 4-Kinase
Dialysis
Anions
Hepatocellular Carcinoma
Catalytic Domain
Cells
Modulation
Ions
Membranes
Antibodies

ASJC Scopus subject areas

  • Biochemistry

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Phosphatidylinositol 3-kinase contributes to cell volume regulation through effects on ATP release. / Feranchak, Andrew P.; Roman, Richard M.; Schwiebert, Erik M.; Fitz, J. Gregory.

In: Journal of Biological Chemistry, Vol. 273, No. 24, 12.06.1998, p. 14906-14911.

Research output: Contribution to journalArticle

Feranchak, Andrew P. ; Roman, Richard M. ; Schwiebert, Erik M. ; Fitz, J. Gregory. / Phosphatidylinositol 3-kinase contributes to cell volume regulation through effects on ATP release. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 24. pp. 14906-14911.
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