Phosphatidylinositol 4-kinase III-β is required for Golgi maintenance and cytokinesis in Trypanosoma brucei

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Abstract

The parasitic protozoan Trypanosoma brucei contains two type III phosphatidylinositol 4-kinases (α and β). We have cloned the gene encoding the T. brucei type III phosphatidylinositol 4-kinase β (TbPI4KIII-β), expressed the protein in COS-7 cells, and confirmed that the protein catalyzes the phosphorylation of phosphatidylinositol. Depletion of TbPI4KIII-β in procyclic T. brucei by RNA interference (RNAi) resulted in inhibition of cell growth and a distorted cellular morphology. RNAi cells had a distorted Golgi apparatus, and lysosomal and flagellar pocket proteins were mislocalized. Ultrastructural analysis revealed the internal accumulation of a heterogeneous population of vesicles, abnormal positioning of organelles, and a loss of cell polarity. Scanning electron microcopy revealed a twisted phenotype, and dividing cells often exhibited a detached daughter flagellum and lacked a cleavage furrow. Cell cycle analysis confirmed that cells depleted of TbPI4KIII-β have a postmitotic cytokinesis block that occurs after a single round of mitosis, suggestive of a specific cell cycle block. In summary, TbPI4KIII-β is an essential protein in procyclic T. brucei, required for maintenance of Golgi structure, protein trafficking, normal cellular shape, and cytokinesis.

Original languageEnglish (US)
Pages (from-to)1108-1118
Number of pages11
JournalEukaryotic Cell
Volume6
Issue number7
DOIs
StatePublished - Jul 2007

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1-phosphatidylinositol 4-kinase
Trypanosoma brucei
Trypanosoma brucei brucei
1-Phosphatidylinositol 4-Kinase
Cytokinesis
cytokinesis
Maintenance
RNA Interference
RNA interference
cell cycle
Cell Cycle
Proteins
cells
Cell Polarity
Flagella
protein transport
proteins
protein phosphorylation
COS Cells
phosphatidylinositols

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Phosphatidylinositol 4-kinase III-β is required for Golgi maintenance and cytokinesis in Trypanosoma brucei. / Rodgers, Melissa J.; Albanesi, Joseph P.; Phillips, Margaret A.

In: Eukaryotic Cell, Vol. 6, No. 7, 07.2007, p. 1108-1118.

Research output: Contribution to journalArticle

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