Phosphatidylserine externalization and membrane blebbing are involved in the nonclassical export of FGF1

Aleksandr Kirov, Huda Al-Hashimi, Phil Solomon, Courtney Mazur, Philip E. Thorpe, Peter J. Sims, Francesca Tarantini, Thallapuranam K Suresh Kumar, Igor Prudovsky

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The mechanisms of nonclassical export of signal peptide-less proteins remain insufficiently understood. Here, we demonstrate that stressinduced unconventional export of FGF1, a potent and ubiquitously expressed mitogenic and proangiogenic protein, is associated with and dependent on the formation of membrane blebs and localized cell surface exposure of phosphatidylserine (PS). In addition, we found that the differentiation of promonocytic cells results in massive FGF1 release, which also correlates with membrane blebbing and exposure of PS. These findings indicate that the externalization of acidic phospholipids could be used as a pharmacological target to regulate the availability of FGF1 in the organism.

Original languageEnglish (US)
Pages (from-to)956-966
Number of pages11
JournalJournal of Cellular Biochemistry
Volume113
Issue number3
DOIs
StatePublished - Mar 1 2012

Keywords

  • Blebbing
  • Fgf1
  • Nonclassical Secretion
  • Phosphatidylserine Exposure
  • Plscr1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kirov, A., Al-Hashimi, H., Solomon, P., Mazur, C., Thorpe, P. E., Sims, P. J., Tarantini, F., Kumar, T. K. S., & Prudovsky, I. (2012). Phosphatidylserine externalization and membrane blebbing are involved in the nonclassical export of FGF1. Journal of Cellular Biochemistry, 113(3), 956-966. https://doi.org/10.1002/jcb.23425