TY - JOUR
T1 - Phospholipid composition of lamellar bodies formed by fetal rabbit lung type II cells in organ culture
AU - Longmuir, Kenneth J.
AU - Snyder, Jeanne M.
AU - Mendelson, Carole R.
AU - Johnston, John M.
N1 - Funding Information:
‘This investigation was supported in part by grants from USPHS lPOl-HD13912, The Robert A. Welch Foundation, Houston, Texas, and a grant-in-aid from the Dallas Crystal Charity Ball. ’ Recipient of a USPHS Pulmonary Faculty Training Award (5-K08-HL66456) sponsored by the Department of Physiology and Biophysics, California College of Medicine, University of California, Irvine. 3 To whom all correspondence should be addressed at the Department of Biochemistry, University Texas Health Science Center.
PY - 1981/12
Y1 - 1981/12
N2 - Lung tissue obtained from fetal rabbits of 23 days gestational age was maintained in organ culture to study the in vitro formation of lamellar body phospholipids. During the culture period, the epithelium of the prealveolar ducts of the explants differentiated to form type II pneumonocytes. After 8 days in culture, the explants were harvested, homogenized, and two lamellar body fractions were isolated by sucrose density gradient centrifugation. The lamellar body fraction which best retained the distinct multilamellar structure was recovered at the interface between a solution of buffer without sucrose and buffer containing 0.41 m sucrose. The phospholipid compositions of both lamellar body fractions were similar to those reported for lamellar bodies and surfactant isolated from fetal rabbit lung, with the exception of a slightly higher phosphatidylethanolamine content. The disaturated phosphatidylcholine content of the lamellar body fractions, expressed as a percentage of total lipid phosphorus, was not influenced by the presence of palmitate in the medium.
AB - Lung tissue obtained from fetal rabbits of 23 days gestational age was maintained in organ culture to study the in vitro formation of lamellar body phospholipids. During the culture period, the epithelium of the prealveolar ducts of the explants differentiated to form type II pneumonocytes. After 8 days in culture, the explants were harvested, homogenized, and two lamellar body fractions were isolated by sucrose density gradient centrifugation. The lamellar body fraction which best retained the distinct multilamellar structure was recovered at the interface between a solution of buffer without sucrose and buffer containing 0.41 m sucrose. The phospholipid compositions of both lamellar body fractions were similar to those reported for lamellar bodies and surfactant isolated from fetal rabbit lung, with the exception of a slightly higher phosphatidylethanolamine content. The disaturated phosphatidylcholine content of the lamellar body fractions, expressed as a percentage of total lipid phosphorus, was not influenced by the presence of palmitate in the medium.
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U2 - 10.1016/0003-9861(81)90392-1
DO - 10.1016/0003-9861(81)90392-1
M3 - Article
C2 - 7325674
AN - SCOPUS:0019821125
SN - 0003-9861
VL - 212
SP - 491
EP - 500
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -