TY - JOUR
T1 - Phosphorylation of PERIOD is influenced by cycling physical associations of DOUBLE-TIME, PERIOD, and TIMELESS in the Drosophila clock
AU - Kloss, Brian
AU - Rothenfluh, Adrian
AU - Young, Michael W.
AU - Saez, Lino
N1 - Funding Information:
We thank Cedric Wesley for help with the IPs, Akira Matsumoto for suggesting the use of TSA and for help with cryosections, Thomas Moran for generously providing anti-HA antibody, and Ralf Stanewsky and Jeff Hall for anti-PER and anti-PDF antibodies. This work was funded by NIH GM 54339, and the NSF Science and Technology Center for Biological Timing (M.W.Y.).
PY - 2001
Y1 - 2001
N2 - The clock gene double-time (dbt) encodes an ortholog of casein kinase Iε that promotes phosphorylation and turnover of the PERIOD protein. Whereas the period (per), timeless (tim), and dClock (dClk) genes of Drosophila each contribute cycling mRNA and protein to a circadian clock, dbt RNA and DBT protein are constitutively expressed. Robust circadian changes in DBT subcellular localization are nevertheless observed in clock-containing cells of the fly head. These localization rhythms accompany formation of protein complexes that include PER, TIM, and DBT, and reflect periodic redistribution between the nucleus and the cytoplasm. Nuclear phosphorylation of PER is strongly enhanced when TIM is removed from PER/TIM/DBT complexes. The varying associations of PER, DBT and TIM appear to determine the onset and duration of nuclear PER function within the Drosophila clock.
AB - The clock gene double-time (dbt) encodes an ortholog of casein kinase Iε that promotes phosphorylation and turnover of the PERIOD protein. Whereas the period (per), timeless (tim), and dClock (dClk) genes of Drosophila each contribute cycling mRNA and protein to a circadian clock, dbt RNA and DBT protein are constitutively expressed. Robust circadian changes in DBT subcellular localization are nevertheless observed in clock-containing cells of the fly head. These localization rhythms accompany formation of protein complexes that include PER, TIM, and DBT, and reflect periodic redistribution between the nucleus and the cytoplasm. Nuclear phosphorylation of PER is strongly enhanced when TIM is removed from PER/TIM/DBT complexes. The varying associations of PER, DBT and TIM appear to determine the onset and duration of nuclear PER function within the Drosophila clock.
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U2 - 10.1016/S0896-6273(01)00320-8
DO - 10.1016/S0896-6273(01)00320-8
M3 - Article
C2 - 11430804
AN - SCOPUS:0034964474
SN - 0896-6273
VL - 30
SP - 699
EP - 706
JO - Neuron
JF - Neuron
IS - 3
ER -