Phosphorylation of spore coat proteins by a family of atypical protein kinases

Kim B. Nguyen, Anju Sreelatha, Eric S. Durrant, Javier Lopez-Garrido, Anna Muszewska, Małgorzata Dudkiewicz, Marcin Grynberg, Samantha Yee, Kit Pogliano, Diana R. Tomchick, Krzysztof Pawłowski, Jack E. Dixon, Vincent S. Tagliabracci

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins. One member, family with sequence similarity 20C (Fam20C), is the physiological Golgi casein kinase. While examining distantly related protein sequences, we observed low levels of identity between the spore coat protein H (CotH), and the Fam20C-related secretory pathway kinases. CotH is a component of the spore in many bacterial and eukaryotic species, and is required for efficient germination of spores in Bacillus subtilis; however, the mechanism by which CotH affects germination is unclear. Here, we show that CotH is a protein kinase. The crystal structure of CotH reveals an atypical protein kinase-like fold with a unique mode of ATP binding. Examination of the genes neighboring cotH in B. subtilis led us to identify two spore coat proteins, CotB and CotG, as CotH substrates. Furthermore, we show that CotH-dependent phosphorylation of CotB and CotG is required for the efficient germination of B. subtilis spores. Collectively, our results define a family of atypical protein kinases and reveal an unexpected role for protein phosphorylation in spore biology.

Original languageEnglish (US)
Pages (from-to)E3482-E3491
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number25
DOIs
StatePublished - Jun 21 2016

Fingerprint

Capsid Proteins
Spores
Protein Kinases
Phosphorylation
Germination
Bacillus subtilis
Secretory Pathway
Proteins
Phosphotransferases
Casein Kinases
Adenosine Triphosphate
Enzymes
Genes

Keywords

  • CotB
  • CotG
  • CotH
  • Kinase
  • Phosphorylation

ASJC Scopus subject areas

  • General

Cite this

Phosphorylation of spore coat proteins by a family of atypical protein kinases. / Nguyen, Kim B.; Sreelatha, Anju; Durrant, Eric S.; Lopez-Garrido, Javier; Muszewska, Anna; Dudkiewicz, Małgorzata; Grynberg, Marcin; Yee, Samantha; Pogliano, Kit; Tomchick, Diana R.; Pawłowski, Krzysztof; Dixon, Jack E.; Tagliabracci, Vincent S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 25, 21.06.2016, p. E3482-E3491.

Research output: Contribution to journalArticle

Nguyen, KB, Sreelatha, A, Durrant, ES, Lopez-Garrido, J, Muszewska, A, Dudkiewicz, M, Grynberg, M, Yee, S, Pogliano, K, Tomchick, DR, Pawłowski, K, Dixon, JE & Tagliabracci, VS 2016, 'Phosphorylation of spore coat proteins by a family of atypical protein kinases', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 25, pp. E3482-E3491. https://doi.org/10.1073/pnas.1605917113
Nguyen, Kim B. ; Sreelatha, Anju ; Durrant, Eric S. ; Lopez-Garrido, Javier ; Muszewska, Anna ; Dudkiewicz, Małgorzata ; Grynberg, Marcin ; Yee, Samantha ; Pogliano, Kit ; Tomchick, Diana R. ; Pawłowski, Krzysztof ; Dixon, Jack E. ; Tagliabracci, Vincent S. / Phosphorylation of spore coat proteins by a family of atypical protein kinases. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 25. pp. E3482-E3491.
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