Phosphorylation of the Gal4 DNA-binding domain is essential for activator mono-ubiquitylation and efficient promoter occupancy

Anwarul Ferdous, Melissa O'Neal, Kip Nalley, Devanjan Sikder, Thomas Kodadek, Stephen Albert Johnston

Research output: Contribution to journalArticle

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Abstract

Recent analysis of a Gal4 mutant (Gap71) carrying three point mutations (S22D, K23Q and K25F) in its DNA-binding domain (DBD), has demonstrated that it cannot occupy GAL promoters efficiently in cells and that it is not mono-ubiquitylated, suggesting a functional link between this modification and stable DNA binding in cells. The mechanistic underpinning of this phenotype is that this protein is hypersensitive to a newly discovered activity of the proteasomal ATPases-their ability to actively dissociate transcription factor-DNA complexes after direct interaction with the activation domain. In this paper, we examine the roles of each of the three point mutations contained in Gap71 individually. These experiments have revealed that serine 22 is a site of phosphorylation in the Gal4 DBD and that lysine 23 is essential for S22 phosphorylation, possibly acting as part of the kinase recognition site. Mutation of either residue blocks Gal4 DBD phosphorylation, its subsequent ubiquitylation and compromises the ability of the activator to bind promoter DNA in vivo. These data represent the first report of an essential phosphorylation event that is critical for the activity of this paradigmatic transcription factor.

Original languageEnglish (US)
Pages (from-to)1116-1125
Number of pages10
JournalMolecular BioSystems
Volume4
Issue number11
DOIs
StatePublished - 2008

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Ubiquitination
Phosphorylation
DNA
Point Mutation
Transcription Factors
Serine
Lysine
Adenosine Triphosphatases
Phosphotransferases
Phenotype
Mutation
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Phosphorylation of the Gal4 DNA-binding domain is essential for activator mono-ubiquitylation and efficient promoter occupancy. / Ferdous, Anwarul; O'Neal, Melissa; Nalley, Kip; Sikder, Devanjan; Kodadek, Thomas; Johnston, Stephen Albert.

In: Molecular BioSystems, Vol. 4, No. 11, 2008, p. 1116-1125.

Research output: Contribution to journalArticle

Ferdous, Anwarul ; O'Neal, Melissa ; Nalley, Kip ; Sikder, Devanjan ; Kodadek, Thomas ; Johnston, Stephen Albert. / Phosphorylation of the Gal4 DNA-binding domain is essential for activator mono-ubiquitylation and efficient promoter occupancy. In: Molecular BioSystems. 2008 ; Vol. 4, No. 11. pp. 1116-1125.
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