Photo-activatable Cre recombinase regulates gene expression in vivo

Suzanne E. Schindler, Jordan G. McCall, Ping Yan, Krzystof L. Hyrc, Mingjie Li, Chandra L. Tucker, Jin Moo Lee, Michael R. Bruchas, Marc I. Diamond

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.

Original languageEnglish (US)
Article number13627
JournalScientific Reports
Volume5
DOIs
StatePublished - Sep 9 2015

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Gene Expression
Lighting
Brain
Optogenetics
Optical Fibers
Light
Photons
Skull
Hippocampus
Cre recombinase
Genes

ASJC Scopus subject areas

  • General

Cite this

Schindler, S. E., McCall, J. G., Yan, P., Hyrc, K. L., Li, M., Tucker, C. L., ... Diamond, M. I. (2015). Photo-activatable Cre recombinase regulates gene expression in vivo. Scientific Reports, 5, [13627]. https://doi.org/10.1038/srep13627

Photo-activatable Cre recombinase regulates gene expression in vivo. / Schindler, Suzanne E.; McCall, Jordan G.; Yan, Ping; Hyrc, Krzystof L.; Li, Mingjie; Tucker, Chandra L.; Lee, Jin Moo; Bruchas, Michael R.; Diamond, Marc I.

In: Scientific Reports, Vol. 5, 13627, 09.09.2015.

Research output: Contribution to journalArticle

Schindler, SE, McCall, JG, Yan, P, Hyrc, KL, Li, M, Tucker, CL, Lee, JM, Bruchas, MR & Diamond, MI 2015, 'Photo-activatable Cre recombinase regulates gene expression in vivo', Scientific Reports, vol. 5, 13627. https://doi.org/10.1038/srep13627
Schindler SE, McCall JG, Yan P, Hyrc KL, Li M, Tucker CL et al. Photo-activatable Cre recombinase regulates gene expression in vivo. Scientific Reports. 2015 Sep 9;5. 13627. https://doi.org/10.1038/srep13627
Schindler, Suzanne E. ; McCall, Jordan G. ; Yan, Ping ; Hyrc, Krzystof L. ; Li, Mingjie ; Tucker, Chandra L. ; Lee, Jin Moo ; Bruchas, Michael R. ; Diamond, Marc I. / Photo-activatable Cre recombinase regulates gene expression in vivo. In: Scientific Reports. 2015 ; Vol. 5.
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