Photo-activatable Cre recombinase regulates gene expression in vivo

Suzanne E. Schindler, Jordan G. McCall, Ping Yan, Krzystof L. Hyrc, Mingjie Li, Chandra L. Tucker, Jin Moo Lee, Michael R. Bruchas, Marc I. Diamond

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.

Original languageEnglish (US)
Article number13627
JournalScientific reports
Volume5
DOIs
StatePublished - Sep 9 2015

ASJC Scopus subject areas

  • General

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