Two proteins have been identified in rat liver plasma membranes that bind a photoreactive GTP analogue, [32P]γ-azidoanilido GTP, in response to incubation with the Ca2+-mobilizing agonist, vasopressin. The labeled proteins possess apparent molecular masses of 42 and 43 kDa. Their labeling requires Mg2+ and can be inhibited by GTP, its analogues, and GDP but not by other nucleotides. Vasopressin-stimulated labeling is attenuated by a V1 receptor-selective antagonist. The concentration of vasopressin required to stimulate labeling is in the same range (EC50 = 4 nM) as that required for activation of GTPase and phosphoinositide-specific phospholipase C activities in liver plasma membranes. Immunodetection and immunoprecipitation of the [32P]γ-azidoanilido GTP-labeled 42- and 43-kDa proteins with antisera raised against peptide sequences in α(q) indicate that these proteins are members of the recently described G(q) class of G proteins.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|State||Published - 1991|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology