Photoaffinity probes for studying carbohydrate biology

Seok Ho Yu, Amberlyn M. Wands, Jennifer J. Kohler

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Carbohydrates and carbohydrate-containing biomolecules engage in binding events that underlie many essential biological processes. Yet these carbohydrate-mediated interactions are often poorly characterized, due to their low affinities and heterogenous natures. The use of photocrosslinking functional groups offers a way to photochemically capture carbohydrate-containing complexes, which can be isolated for further analysis. Here we survey progress in the synthesis and use of carbohydrate-based photoprobes, reagents that incorporate carbohydrates or their analogs, photocrosslinking moieties, and affinity purification handles. Carbohydrate photoprobes, used in combination with modern mass spectrometry methods, can provide important new insights into the cellular roles of carbohydrates and glycosylated molecules.

Original languageEnglish (US)
Pages (from-to)325-352
Number of pages28
JournalJournal of Carbohydrate Chemistry
Volume31
Issue number4-6
DOIs
StatePublished - 2012

Keywords

  • Aryl azide
  • Benzophenone
  • Crosslinking
  • Diazirine
  • Glycolipid
  • Photoaffinity

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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