Photocrosslinking O-GlcNAcylated Proteins to Neighboring Biomolecules

Emanuela Capota, Han Wu, Jennifer J. Kohler

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This protocol enables identification of the interaction partners of O-GlcNAcylated proteins. The method involves the introduction of the diazirine photocrosslinker onto the O-GlcNAc modification within living cells. The photocrosslinker is activated by UV light to yield covalent crosslinking between O-GlcNAcylated proteins and neighboring molecules. The binding partners can be further characterized by immunoblot or proteomics mass spectrometry methods. The benefits of using the photocrosslinker include the capacity to trap low-affinity binding interactions and the ability to selectively target the interaction partners of the O-GlcNAcylated form of the protein of interest.

Original languageEnglish (US)
Article numbere201
JournalCurrent Protocols
Volume1
Issue number7
DOIs
StatePublished - Jul 2021

Keywords

  • O-GlcNAc
  • diazirine
  • glycosylation
  • metabolic labeling
  • photocrosslinking

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Health Informatics
  • Medical Laboratory Technology
  • Medicine(all)

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