Abstract
This protocol enables identification of the interaction partners of O-GlcNAcylated proteins. The method involves the introduction of the diazirine photocrosslinker onto the O-GlcNAc modification within living cells. The photocrosslinker is activated by UV light to yield covalent crosslinking between O-GlcNAcylated proteins and neighboring molecules. The binding partners can be further characterized by immunoblot or proteomics mass spectrometry methods. The benefits of using the photocrosslinker include the capacity to trap low-affinity binding interactions and the ability to selectively target the interaction partners of the O-GlcNAcylated form of the protein of interest.
Original language | English (US) |
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Article number | e201 |
Journal | Current Protocols |
Volume | 1 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2021 |
Keywords
- O-GlcNAc
- diazirine
- glycosylation
- metabolic labeling
- photocrosslinking
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- Pharmacology, Toxicology and Pharmaceutics(all)
- General Neuroscience
- General Immunology and Microbiology
- Health Informatics
- Medical Laboratory Technology
- General Medicine