Photocrosslinking probes for capture of carbohydrate interactions

Research output: Contribution to journalReview article

Abstract

Glycan-mediated interactions are essential in many biological processes and regulate a wide variety of cellular functions. However, characterizing these interactions is difficult because glycan biosynthesis is not template driven and because carbohydrate recognition events are usually of low affinity and transient. Photocrosslinking carbohydrate probes can form a covalent bond with molecules in close proximity on UV irradiation and are capable of capturing interactions between glycans and glycan-binding proteins in situ. Because of these advantages, multiple photocrosslinking carbohydrate probes have been designed and applied to study the biological functions of glycans. This review will discuss recent advances in the development of novel photocrosslinking functional groups and the design of photocrosslinking probes to detect interactions mediated by glycolipids, peptidoglycan, and multivalent carbohydrate ligands. These probes have demonstrated the potential to address some of the major challenges in the study of glycan-mediated interactions in both model systems and in more complex biological settings.

Original languageEnglish (US)
Pages (from-to)173-182
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume53
DOIs
StatePublished - Dec 2019

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Polysaccharides
Carbohydrates
Biological Phenomena
Covalent bonds
Peptidoglycan
Glycolipids
Biosynthesis
Functional groups
Carrier Proteins
Irradiation
Ligands
Molecules

Keywords

  • Bioorthogonal
  • Diazirine
  • Glycolipids
  • Glycoproteins
  • Glycosylation
  • Peptidoglycan
  • Photocrosslinking

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Photocrosslinking probes for capture of carbohydrate interactions. / Wu, Han; Kohler, Jennifer.

In: Current Opinion in Chemical Biology, Vol. 53, 12.2019, p. 173-182.

Research output: Contribution to journalReview article

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