Photocrosslinking sugars for capturing glycan-dependent interactions

Akiko Fujita, Jennifer J. Kohler

Research output: Contribution to journalArticle

Abstract

Photocrosslinking approaches are an important strategy to covalently capture biomolecular interactions. Covalent capture by photocrosslinking is particularly powerful for glycan-dependent interactions because these binding events are typically low-affinity and unable to withstand common purification protocols. Implementation of photocrosslinking methods is based on the incorporation of a photoactivatable crosslinking functional group onto glycan-containing molecules. Upon activation with UV radiation, the photocrosslinking group is converted to a reactive species, which forms a covalent bond with neighboring molecules. Resulting crosslinked complexes can be analyzed by immunological and mass spectrometry techniques to identify glycan-dependent binding partners. In some cases, photocrosslinker incorporation can be achieved in living cells, enabling detection of glycan-dependent interactions in their native context. In this mini-review, we describe new sugar-based photocrosslinking reagents and illustrate how they have been used to photochemically characterize glycan-dependent interactions.

Original languageEnglish (US)
Pages (from-to)E1-E7
JournalTrends in Glycoscience and Glycotechnology
Volume27
Issue number156
DOIs
StatePublished - Jul 24 2015

Fingerprint

Sugars
Polysaccharides
Molecules
Covalent bonds
Ultraviolet radiation
Crosslinking
Functional groups
Purification
Mass spectrometry
Mass Spectrometry
Chemical activation
Cells
Radiation

Keywords

  • Metabolic engineering
  • O-GlcNAc
  • Photocrosslinking
  • Sialic acid
  • Sugar-dependent interaction

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry

Cite this

Photocrosslinking sugars for capturing glycan-dependent interactions. / Fujita, Akiko; Kohler, Jennifer J.

In: Trends in Glycoscience and Glycotechnology, Vol. 27, No. 156, 24.07.2015, p. E1-E7.

Research output: Contribution to journalArticle

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