Physical and functional interactions of monoubiquitylated transactivators with the proteasome

Chase T. Archer, Lyle Burdine, Bo Liu, Anwarul Ferdous, Stephen Albert Johnston, Thomas Kodadek

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Destabilization of activator-DNA complexes by the proteasomal ATPases can inhibit transcription by limiting activator interaction with DNA. Modification of the activator by monoubiquitylation protects the activator from this destabilization activity. In this study, we probe the mechanism of this protective effect of monoubiquitylation. Using novel label transfer and chemical cross-linking techniques, we show that ubiquitin contacts the ATPase complex directly, apparently via Rpn1 and Rpt1. This interaction results in the dissociation of the activation domain-ATPase complex via an allosteric process. A model is proposed in which activator monoubiquitylation serves to limit the lifetime of the activator-ATPase complex interaction and thus the ability of the ATPases to unfold the activator and dissociate the protein-DNA complex.

Original languageEnglish (US)
Pages (from-to)21789-21798
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number31
DOIs
StatePublished - Aug 1 2008

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Trans-Activators
Proteasome Endopeptidase Complex
Adenosine Triphosphatases
DNA
Transcription
Ubiquitin
Labels
Chemical activation
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Physical and functional interactions of monoubiquitylated transactivators with the proteasome. / Archer, Chase T.; Burdine, Lyle; Liu, Bo; Ferdous, Anwarul; Johnston, Stephen Albert; Kodadek, Thomas.

In: Journal of Biological Chemistry, Vol. 283, No. 31, 01.08.2008, p. 21789-21798.

Research output: Contribution to journalArticle

Archer, Chase T. ; Burdine, Lyle ; Liu, Bo ; Ferdous, Anwarul ; Johnston, Stephen Albert ; Kodadek, Thomas. / Physical and functional interactions of monoubiquitylated transactivators with the proteasome. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 31. pp. 21789-21798.
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