Physiological analysis of Methylobacterium extorquens AM1 grown in continuous and batch cultures

Xiaofeng Guo, Mary E. Lidstrom

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Chemostat cultures of Methylobacterium extorquens AM1 grown on methanol or succinate at a range of dilution rates were compared to batch cultures in terms of enzyme levels, poly-β-hydroxybutyrate content, and intracellular concentrations of adenine and pyridine nucleotides. In both chemostat and batch cultures, enzymes specific to C1 metabolism were up-regulated during growth on methanol and down-regulated during growth on succinate, polyhydroxybutyrate levels were higher on succinate, intracellular ATP levels and the energy charge were higher during growth on methanol, while the pools of reducing equivalents were higher during growth on succinate. For most of the tested parameters, little alteration occurred in response to growth rate. Overall, we conclude that the chemostat cultivation conditions developed in this study roughly mimic the growth in batch cultures, but provide a better control over the culturing conditions and a better data reproducibility, which are important for integrative functional studies. This study provides baseline data for future work using chemostat cultures, defining key similarities and differences in the physiology compared to existing batch culture data.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalArchives of Microbiology
Volume186
Issue number2
DOIs
StatePublished - Aug 1 2006

Keywords

  • Enzyme activities
  • Methylobacterium extorquens AM1
  • Methylotroph
  • Nucleotides
  • PHB
  • Physiology

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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