Physiological concentrations of prolactin can promote the growth of human breast tumor cells in culture

W. B. Malarkey, M. Kennedy, L. E. Allred, G. Milo

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

There is only indirect evidence at present to suggest a role for PRL in either the genesis or progression of human breast cancer. Here, we report the results of experiments in primary cultures of breast tumor cells from a hyperprolactinemic breast cancer patient who had an elevated mean 24-h PRL concentration but a normal diurnal variation of PRL release. The effects of PRL and GH on the growth of the dispersed cells from the breast tumor was evaluated in monolayer culture using a recently developed microculture technique. Pharmacological quantities of GH produced significant increases in the number of population doublings of the breast tumor cells. Also, PRL concentrations present in the patient's circulation were demonstrated to significantly increase the number of population doublings of the breast tumor cells obtained in primary cultures. Thus, physiological concentrations of PRL stimulated the growth of breast tumor cells from this premenopausal patient.

Original languageEnglish (US)
Pages (from-to)673-677
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume56
Issue number4
StatePublished - 1983

Fingerprint

Cell culture
Prolactin
Tumors
Cell Culture Techniques
Cells
Breast Neoplasms
Growth
Monolayers
Population
Pharmacology
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Physiological concentrations of prolactin can promote the growth of human breast tumor cells in culture. / Malarkey, W. B.; Kennedy, M.; Allred, L. E.; Milo, G.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 56, No. 4, 1983, p. 673-677.

Research output: Contribution to journalArticle

Malarkey, W. B. ; Kennedy, M. ; Allred, L. E. ; Milo, G. / Physiological concentrations of prolactin can promote the growth of human breast tumor cells in culture. In: Journal of Clinical Endocrinology and Metabolism. 1983 ; Vol. 56, No. 4. pp. 673-677.
@article{b7e30d74676b4a8daee5c906327d9b83,
title = "Physiological concentrations of prolactin can promote the growth of human breast tumor cells in culture",
abstract = "There is only indirect evidence at present to suggest a role for PRL in either the genesis or progression of human breast cancer. Here, we report the results of experiments in primary cultures of breast tumor cells from a hyperprolactinemic breast cancer patient who had an elevated mean 24-h PRL concentration but a normal diurnal variation of PRL release. The effects of PRL and GH on the growth of the dispersed cells from the breast tumor was evaluated in monolayer culture using a recently developed microculture technique. Pharmacological quantities of GH produced significant increases in the number of population doublings of the breast tumor cells. Also, PRL concentrations present in the patient's circulation were demonstrated to significantly increase the number of population doublings of the breast tumor cells obtained in primary cultures. Thus, physiological concentrations of PRL stimulated the growth of breast tumor cells from this premenopausal patient.",
author = "Malarkey, {W. B.} and M. Kennedy and Allred, {L. E.} and G. Milo",
year = "1983",
language = "English (US)",
volume = "56",
pages = "673--677",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Physiological concentrations of prolactin can promote the growth of human breast tumor cells in culture

AU - Malarkey, W. B.

AU - Kennedy, M.

AU - Allred, L. E.

AU - Milo, G.

PY - 1983

Y1 - 1983

N2 - There is only indirect evidence at present to suggest a role for PRL in either the genesis or progression of human breast cancer. Here, we report the results of experiments in primary cultures of breast tumor cells from a hyperprolactinemic breast cancer patient who had an elevated mean 24-h PRL concentration but a normal diurnal variation of PRL release. The effects of PRL and GH on the growth of the dispersed cells from the breast tumor was evaluated in monolayer culture using a recently developed microculture technique. Pharmacological quantities of GH produced significant increases in the number of population doublings of the breast tumor cells. Also, PRL concentrations present in the patient's circulation were demonstrated to significantly increase the number of population doublings of the breast tumor cells obtained in primary cultures. Thus, physiological concentrations of PRL stimulated the growth of breast tumor cells from this premenopausal patient.

AB - There is only indirect evidence at present to suggest a role for PRL in either the genesis or progression of human breast cancer. Here, we report the results of experiments in primary cultures of breast tumor cells from a hyperprolactinemic breast cancer patient who had an elevated mean 24-h PRL concentration but a normal diurnal variation of PRL release. The effects of PRL and GH on the growth of the dispersed cells from the breast tumor was evaluated in monolayer culture using a recently developed microculture technique. Pharmacological quantities of GH produced significant increases in the number of population doublings of the breast tumor cells. Also, PRL concentrations present in the patient's circulation were demonstrated to significantly increase the number of population doublings of the breast tumor cells obtained in primary cultures. Thus, physiological concentrations of PRL stimulated the growth of breast tumor cells from this premenopausal patient.

UR - http://www.scopus.com/inward/record.url?scp=0020700098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020700098&partnerID=8YFLogxK

M3 - Article

C2 - 6833457

AN - SCOPUS:0020700098

VL - 56

SP - 673

EP - 677

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -