Pias1 is essential for erythroid and vascular development in the mouse embryo

Jerfiz D. Constanzo, Mi Deng Ph.D., Smita Rindhe, Ke jing Tang, Chengcheng Zhang, Pier P Scaglioni

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The protein inhibitor of activated STAT-1 (PIAS1) is one of the few known SUMO E3 ligases. PIAS1 has been implicated in several biological processes including repression of innate immunity and DNA repair. However, PIAS1 function during development and tissue differentiation has not been studied. Here, we report that Pias1 is required for proper embryonic development. Approximately 90% of Pias1 null embryos die in utero between E10.5 and E12.5. We found significant apoptosis within the yolk sac (YS) blood vessels and concomitant loss of red blood cells (RBCs) resulting in profound anemia. In addition, Pias1 loss impairs YS angiogenesis and results in defective capillary plexus formation and blood vessel occlusions. Moreover, heart development is impaired as a result of loss of myocardium muscle mass. Accordingly, we found that Pias1 expression in primary myoblasts enhances the induction of cardiac muscle genes MyoD, Myogenin and Myomaker. PIAS1 protein regulation of cardiac gene transcription is dependent on transcription factors Myocardin and Gata-4. Finally, endothelial cell specific inactivation of Pias1 in vivo impairs YS erythrogenesis, angiogenesis and recapitulates loss of myocardium muscle mass. However, these defects are not sufficient to recapitulate the lethal phenotype of Pias1 null embryos. These findings highlight Pias1 as an essential gene for YS erythropoiesis and vasculogenesis in vivo.

Original languageEnglish (US)
JournalDevelopmental Biology
DOIs
StateAccepted/In press - Nov 17 2015

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Protein Inhibitors of Activated STAT
Yolk Sac
Blood Vessels
Embryonic Structures
Myocardium
Myogenin
Biological Phenomena
Muscles
Ubiquitin-Protein Ligases
Myoblasts
Erythropoiesis
Essential Genes
Innate Immunity
DNA Repair
Genes
Embryonic Development
Anemia
Transcription Factors
Endothelial Cells
Erythrocytes

Keywords

  • Angiogenesis
  • Embryonic development
  • Fetal erythropoiesis
  • Pias1
  • Yolk sac capillary plexus

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Pias1 is essential for erythroid and vascular development in the mouse embryo. / Constanzo, Jerfiz D.; Deng Ph.D., Mi; Rindhe, Smita; Tang, Ke jing; Zhang, Chengcheng; Scaglioni, Pier P.

In: Developmental Biology, 17.11.2015.

Research output: Contribution to journalArticle

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