PICK1

A perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system

Jeff Staudinger, Jumin Zhou, Rob Burgess, Stephen J. Elledge, Eric N. Olson

Research output: Contribution to journalArticle

245 Citations (Scopus)

Abstract

Protein kinase C (PKC) plays a central role in the control of proliferation and differentiation of a wide range of cell types by mediating the signal transduction response to hormones and growth factors. Upon activation by diacylglycerol, PKC translocates to different subcellular sites where it phosphorylates numerous proteins, most of which are unidentified. We used the yeast two-hybrid system to identify proteins that interact with activated PKCα. Using the catalytic region of PKC fused to the DNA binding domain of yeast GAL4 as 'bait' to screen a mouse T cell cDNA library in which cDNA was fused to the GALA activation domain, we cloned several novel proteins that interact with C-kinase (PICKs). One of these proteins, designated PICK1, interacts specifically with the catalytic domain of PKC and is an efficient substrate for phosphorylation by PKC in vitro and in vivo. PICK1 is localized to the perinuclear region and is phosphorylated in response to PKC activation. PICK1 and other PICKs may play important roles in mediating the actions of PKC.

Original languageEnglish (US)
Pages (from-to)263-271
Number of pages9
JournalJournal of Cell Biology
Volume128
Issue number3
DOIs
StatePublished - Feb 1995

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Two-Hybrid System Techniques
Protein Kinase C
Carrier Proteins
Proteins
Catalytic Domain
Phosphotransferases
Diacylglycerol Kinase
Gene Library
Signal Transduction
Intercellular Signaling Peptides and Proteins
Complementary DNA
Yeasts
Phosphorylation
Hormones
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

PICK1 : A perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system. / Staudinger, Jeff; Zhou, Jumin; Burgess, Rob; Elledge, Stephen J.; Olson, Eric N.

In: Journal of Cell Biology, Vol. 128, No. 3, 02.1995, p. 263-271.

Research output: Contribution to journalArticle

Staudinger, Jeff ; Zhou, Jumin ; Burgess, Rob ; Elledge, Stephen J. ; Olson, Eric N. / PICK1 : A perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system. In: Journal of Cell Biology. 1995 ; Vol. 128, No. 3. pp. 263-271.
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