Pig chimeric model with human pluripotent stem cells

Cuiqing Zhong, Jun Wu, Juan Carlos Izpisua Belmonte

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Interspecies chimera formation provides a unique platform for studying donor cell developmental potential, modeling disease in vivo, as well as in vivo production of tissues and organs. The derivation of human pluripotent stem cells (hPSC) from either human embryos or somatic cell reprogramming facilitates our understanding of human development, as well as accelerates our exploration of regenerative medicine for human health. Due to similar organ size, close anatomy, and physiology between pig and human, human-Pig interspecies chimeric model in which pig serves as the host species may open new avenues for studying human embryogenesis, disease pathogenesis, and generation of human organ for transplantation to solve the worldwide donor organ shortage. Our previous study demonstrated chimeric competency of different types of human PSCs in pig host. In this chapter, we introduce our workflow for the generation of human PSCs and analysis of its chimeric contribution to pre- and postimplantation pig embryos.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-124
Number of pages24
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2005
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Blastocyst
  • Chimeric contribution
  • Epiblast stem cells (EpiSCs)
  • Human embryonic stem cells (hESCs)
  • Human induced pluripotent stem cells (hiPSCs)
  • Human pluripotent stem cells (hPSCs)
  • Pig

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zhong, C., Wu, J., & Izpisua Belmonte, J. C. (2019). Pig chimeric model with human pluripotent stem cells. In Methods in Molecular Biology (pp. 101-124). (Methods in Molecular Biology; Vol. 2005). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9524-0_8