TY - CHAP
T1 - Pig chimeric model with human pluripotent stem cells
AU - Zhong, Cuiqing
AU - Wu, Jun
AU - Izpisua Belmonte, Juan Carlos
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Interspecies chimera formation provides a unique platform for studying donor cell developmental potential, modeling disease in vivo, as well as in vivo production of tissues and organs. The derivation of human pluripotent stem cells (hPSC) from either human embryos or somatic cell reprogramming facilitates our understanding of human development, as well as accelerates our exploration of regenerative medicine for human health. Due to similar organ size, close anatomy, and physiology between pig and human, human-Pig interspecies chimeric model in which pig serves as the host species may open new avenues for studying human embryogenesis, disease pathogenesis, and generation of human organ for transplantation to solve the worldwide donor organ shortage. Our previous study demonstrated chimeric competency of different types of human PSCs in pig host. In this chapter, we introduce our workflow for the generation of human PSCs and analysis of its chimeric contribution to pre- and postimplantation pig embryos.
AB - Interspecies chimera formation provides a unique platform for studying donor cell developmental potential, modeling disease in vivo, as well as in vivo production of tissues and organs. The derivation of human pluripotent stem cells (hPSC) from either human embryos or somatic cell reprogramming facilitates our understanding of human development, as well as accelerates our exploration of regenerative medicine for human health. Due to similar organ size, close anatomy, and physiology between pig and human, human-Pig interspecies chimeric model in which pig serves as the host species may open new avenues for studying human embryogenesis, disease pathogenesis, and generation of human organ for transplantation to solve the worldwide donor organ shortage. Our previous study demonstrated chimeric competency of different types of human PSCs in pig host. In this chapter, we introduce our workflow for the generation of human PSCs and analysis of its chimeric contribution to pre- and postimplantation pig embryos.
KW - Blastocyst
KW - Chimeric contribution
KW - Epiblast stem cells (EpiSCs)
KW - Human embryonic stem cells (hESCs)
KW - Human induced pluripotent stem cells (hiPSCs)
KW - Human pluripotent stem cells (hPSCs)
KW - Pig
UR - http://www.scopus.com/inward/record.url?scp=85067050053&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067050053&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9524-0_8
DO - 10.1007/978-1-4939-9524-0_8
M3 - Chapter
C2 - 31175649
AN - SCOPUS:85067050053
T3 - Methods in Molecular Biology
SP - 101
EP - 124
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -