Pineal opsin: A nonvisual opsin expressed in chick pineal

Marianna Max, Peter J. McKinnon, Kenneth J. Seidenman, R. Keith Barrett, Meredithe L. Applebury, Joseph S. Takahashi, Robert F. Margolskee

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Pineal opsin (P-opsin), an opsin from chick that is highly expressed in pineal but is not detectable in retina, was cloned by the polymerase chain reaction. It is likely that the P-opsin lineage diverged from the retinal opsins early in opsin evolution. The amino acid sequence of P-opsin is 42 to 46 percent identical to that of the retinal opsins. P-opsin is a seven-membrane spanning, G protein-linked receptor with a Schiff-base lysine in the seventh membrane span and a Schiff-base counterion in the third membrane span. The primary sequence of P-opsin suggests that it will be maximally sensitive to ∼500-nanometer light and produce a slow and prolonged phototransduction response consistent with the nonvisual function of pineal photoreception.

Original languageEnglish (US)
Pages (from-to)1502-1506
Number of pages5
JournalScience
Volume267
Issue number5203
StatePublished - 1995

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Opsins
Schiff Bases
Membranes
Light Signal Transduction
GTP-Binding Proteins
Lysine
Retina
Amino Acid Sequence
Light
Polymerase Chain Reaction

ASJC Scopus subject areas

  • General

Cite this

Max, M., McKinnon, P. J., Seidenman, K. J., Barrett, R. K., Applebury, M. L., Takahashi, J. S., & Margolskee, R. F. (1995). Pineal opsin: A nonvisual opsin expressed in chick pineal. Science, 267(5203), 1502-1506.

Pineal opsin : A nonvisual opsin expressed in chick pineal. / Max, Marianna; McKinnon, Peter J.; Seidenman, Kenneth J.; Barrett, R. Keith; Applebury, Meredithe L.; Takahashi, Joseph S.; Margolskee, Robert F.

In: Science, Vol. 267, No. 5203, 1995, p. 1502-1506.

Research output: Contribution to journalArticle

Max, M, McKinnon, PJ, Seidenman, KJ, Barrett, RK, Applebury, ML, Takahashi, JS & Margolskee, RF 1995, 'Pineal opsin: A nonvisual opsin expressed in chick pineal', Science, vol. 267, no. 5203, pp. 1502-1506.
Max M, McKinnon PJ, Seidenman KJ, Barrett RK, Applebury ML, Takahashi JS et al. Pineal opsin: A nonvisual opsin expressed in chick pineal. Science. 1995;267(5203):1502-1506.
Max, Marianna ; McKinnon, Peter J. ; Seidenman, Kenneth J. ; Barrett, R. Keith ; Applebury, Meredithe L. ; Takahashi, Joseph S. ; Margolskee, Robert F. / Pineal opsin : A nonvisual opsin expressed in chick pineal. In: Science. 1995 ; Vol. 267, No. 5203. pp. 1502-1506.
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