PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory

Lenora J. Volk, Julia L. Bachman, Richard Johnson, Yilin Yu, Richard L. Huganir

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Long-term potentiation (LTP), a well-characterized form of synaptic plasticity, has long been postulated as a cellular correlate of learning and memory. Although LTP can persist for long periods of time, the mechanisms underlying LTP maintenance, in the midst of ongoing protein turnover and synaptic activity, remain elusive. Sustained activation of the brain-specific protein kinase C (PKC) isoform protein kinase M-ζ (PKM-ζ) has been reported to be necessary for both LTP maintenance and long-term memory. Inhibiting PKM-ζ activity using a synthetic zeta inhibitory peptide (ZIP) based on the PKC-ζ pseudosubstrate sequence reverses established LTP in vitro and in vivo. More notably, infusion of ZIP eliminates memories for a growing list of experience-dependent behaviours, including active place avoidance, conditioned taste aversion, fear conditioning and spatial learning. However, most of the evidence supporting a role for PKM-ζ in LTP and memory relies heavily on pharmacological inhibition of PKM-ζ by ZIP. To further investigate the involvement of PKM-ζ in the maintenance of LTP and memory, we generated transgenic mice lacking PKC-ζ and PKM-ζ. We find that both conventional and conditional PKC-ζ/PKM-ζ knockout mice show normal synaptic transmission and LTP at Schaffer collateral-CA1 synapses, and have no deficits in several hippocampal-dependent learning and memory tasks. Notably, ZIP still reverses LTP in PKC-ζ/PKM-ζ knockout mice, indicating that the effects of ZIP are independent of PKM-ζ.

Original languageEnglish (US)
Pages (from-to)420-423
Number of pages4
JournalNature
Volume493
Issue number7432
DOIs
StatePublished - Jan 17 2013

Fingerprint

Neuronal Plasticity
Long-Term Potentiation
Protein Kinase C
Learning
Long-Term Memory
Peptides
Maintenance
Knockout Mice
EphA5 Receptor
Synaptic Transmission
Synapses
Transgenic Mice
Fear
Hippocampus
Protein Isoforms
Pharmacology

ASJC Scopus subject areas

  • General

Cite this

Volk, L. J., Bachman, J. L., Johnson, R., Yu, Y., & Huganir, R. L. (2013). PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory. Nature, 493(7432), 420-423. https://doi.org/10.1038/nature11802

PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory. / Volk, Lenora J.; Bachman, Julia L.; Johnson, Richard; Yu, Yilin; Huganir, Richard L.

In: Nature, Vol. 493, No. 7432, 17.01.2013, p. 420-423.

Research output: Contribution to journalArticle

Volk, LJ, Bachman, JL, Johnson, R, Yu, Y & Huganir, RL 2013, 'PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory', Nature, vol. 493, no. 7432, pp. 420-423. https://doi.org/10.1038/nature11802
Volk, Lenora J. ; Bachman, Julia L. ; Johnson, Richard ; Yu, Yilin ; Huganir, Richard L. / PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory. In: Nature. 2013 ; Vol. 493, No. 7432. pp. 420-423.
@article{7ea09ad8a8ef45a68e97dcadac2c8c7e,
title = "PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory",
abstract = "Long-term potentiation (LTP), a well-characterized form of synaptic plasticity, has long been postulated as a cellular correlate of learning and memory. Although LTP can persist for long periods of time, the mechanisms underlying LTP maintenance, in the midst of ongoing protein turnover and synaptic activity, remain elusive. Sustained activation of the brain-specific protein kinase C (PKC) isoform protein kinase M-ζ (PKM-ζ) has been reported to be necessary for both LTP maintenance and long-term memory. Inhibiting PKM-ζ activity using a synthetic zeta inhibitory peptide (ZIP) based on the PKC-ζ pseudosubstrate sequence reverses established LTP in vitro and in vivo. More notably, infusion of ZIP eliminates memories for a growing list of experience-dependent behaviours, including active place avoidance, conditioned taste aversion, fear conditioning and spatial learning. However, most of the evidence supporting a role for PKM-ζ in LTP and memory relies heavily on pharmacological inhibition of PKM-ζ by ZIP. To further investigate the involvement of PKM-ζ in the maintenance of LTP and memory, we generated transgenic mice lacking PKC-ζ and PKM-ζ. We find that both conventional and conditional PKC-ζ/PKM-ζ knockout mice show normal synaptic transmission and LTP at Schaffer collateral-CA1 synapses, and have no deficits in several hippocampal-dependent learning and memory tasks. Notably, ZIP still reverses LTP in PKC-ζ/PKM-ζ knockout mice, indicating that the effects of ZIP are independent of PKM-ζ.",
author = "Volk, {Lenora J.} and Bachman, {Julia L.} and Richard Johnson and Yilin Yu and Huganir, {Richard L.}",
year = "2013",
month = "1",
day = "17",
doi = "10.1038/nature11802",
language = "English (US)",
volume = "493",
pages = "420--423",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7432",

}

TY - JOUR

T1 - PKM-ζ is not required for hippocampal synaptic plasticity, learning and memory

AU - Volk, Lenora J.

AU - Bachman, Julia L.

AU - Johnson, Richard

AU - Yu, Yilin

AU - Huganir, Richard L.

PY - 2013/1/17

Y1 - 2013/1/17

N2 - Long-term potentiation (LTP), a well-characterized form of synaptic plasticity, has long been postulated as a cellular correlate of learning and memory. Although LTP can persist for long periods of time, the mechanisms underlying LTP maintenance, in the midst of ongoing protein turnover and synaptic activity, remain elusive. Sustained activation of the brain-specific protein kinase C (PKC) isoform protein kinase M-ζ (PKM-ζ) has been reported to be necessary for both LTP maintenance and long-term memory. Inhibiting PKM-ζ activity using a synthetic zeta inhibitory peptide (ZIP) based on the PKC-ζ pseudosubstrate sequence reverses established LTP in vitro and in vivo. More notably, infusion of ZIP eliminates memories for a growing list of experience-dependent behaviours, including active place avoidance, conditioned taste aversion, fear conditioning and spatial learning. However, most of the evidence supporting a role for PKM-ζ in LTP and memory relies heavily on pharmacological inhibition of PKM-ζ by ZIP. To further investigate the involvement of PKM-ζ in the maintenance of LTP and memory, we generated transgenic mice lacking PKC-ζ and PKM-ζ. We find that both conventional and conditional PKC-ζ/PKM-ζ knockout mice show normal synaptic transmission and LTP at Schaffer collateral-CA1 synapses, and have no deficits in several hippocampal-dependent learning and memory tasks. Notably, ZIP still reverses LTP in PKC-ζ/PKM-ζ knockout mice, indicating that the effects of ZIP are independent of PKM-ζ.

AB - Long-term potentiation (LTP), a well-characterized form of synaptic plasticity, has long been postulated as a cellular correlate of learning and memory. Although LTP can persist for long periods of time, the mechanisms underlying LTP maintenance, in the midst of ongoing protein turnover and synaptic activity, remain elusive. Sustained activation of the brain-specific protein kinase C (PKC) isoform protein kinase M-ζ (PKM-ζ) has been reported to be necessary for both LTP maintenance and long-term memory. Inhibiting PKM-ζ activity using a synthetic zeta inhibitory peptide (ZIP) based on the PKC-ζ pseudosubstrate sequence reverses established LTP in vitro and in vivo. More notably, infusion of ZIP eliminates memories for a growing list of experience-dependent behaviours, including active place avoidance, conditioned taste aversion, fear conditioning and spatial learning. However, most of the evidence supporting a role for PKM-ζ in LTP and memory relies heavily on pharmacological inhibition of PKM-ζ by ZIP. To further investigate the involvement of PKM-ζ in the maintenance of LTP and memory, we generated transgenic mice lacking PKC-ζ and PKM-ζ. We find that both conventional and conditional PKC-ζ/PKM-ζ knockout mice show normal synaptic transmission and LTP at Schaffer collateral-CA1 synapses, and have no deficits in several hippocampal-dependent learning and memory tasks. Notably, ZIP still reverses LTP in PKC-ζ/PKM-ζ knockout mice, indicating that the effects of ZIP are independent of PKM-ζ.

UR - http://www.scopus.com/inward/record.url?scp=84872611798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872611798&partnerID=8YFLogxK

U2 - 10.1038/nature11802

DO - 10.1038/nature11802

M3 - Article

VL - 493

SP - 420

EP - 423

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7432

ER -